PDBsum entry 7u4z

Signaling protein

PDB id: 7u4z

Contents

Protein chain

153 a.a.

Metals

__K

Waters ×40

PDB id:

7u4z

Name:

Signaling protein

Title:

The ubiquitin-associated domain of human thirty-eight negative kinase- 1 flexibly fused to the 1tel crystallization chaperone via a 2- glycine linker and crystallized at very low protein concentration

Structure:

Transcription factor etv6,non-receptor tyrosine-protein kinase tnk1. Chain: a. Synonym: ets translocation variant 6,ets-related protein tel1,tel, cd38 negative kinase 1. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: etv6, tel, tel1, tnk1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.03Å R-factor: 0.203 R-free: 0.232

Authors:

S.Nawarathnage,T.Smith,J.D.Moody

Key ref:

S.Nawarathnage et al. The structure of the human thirty-Eight negative kina ubiquitin-Associated domain determined with and witho fusion to telsam. To be published, . PubMed id: 37776857

Date:

01-Mar-22 Release date: 08-Mar-23

Protein chain

P41212  (ETV6_HUMAN) -  Transcription factor ETV6 from Homo sapiens

Seq: 452 a.a.

Struc: 153 a.a.*

Protein chain

Q13470  (TNK1_HUMAN) -  Non-receptor tyrosine-protein kinase TNK1 from Homo sapiens

Seq: 666 a.a.

Struc: 153 a.a.*

* PDB and UniProt seqs differ at 148 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site