PDBsum entry 7syp

Ribosome

PDB id: 7syp

Contents

Protein chains

217 a.a.

213 a.a.

221 a.a.

228 a.a.

262 a.a.

191 a.a.

237 a.a.

185 a.a.

206 a.a.

185 a.a.

96 a.a.

151 a.a.

117 a.a.

149 a.a.

136 a.a.

120 a.a.

142 a.a.

132 a.a.

144 a.a.

141 a.a.

100 a.a.

83 a.a.

129 a.a.

141 a.a.

124 a.a.

77 a.a.

101 a.a.

83 a.a.

67 a.a.

55 a.a.

57 a.a.

68 a.a.

313 a.a.

25 a.a.

DNA/RNA

Metals

_ZN ×2

PDB id:

7syp

Name:

Ribosome

Title:

Structure of the wt ires and 40s ribosome binary complex, open conformation. Structure 10(wt)

Structure:

18s rrna. Chain: 2. Us2 (sa). Chain: b. Synonym: 40s ribosomal protein sa, 37 kda laminin receptor precursor, 37lrp,37/67 kda laminin receptor,lrp/lr,67 kda laminin receptor,67lr, laminin receptor 1,lamr,laminin-binding protein precursor p40, lbp/p40. Mutation: yes.

Source:

Oryctolagus cuniculus. Organism_taxid: 9986. Rabbit. Hepatitis c virus (isolate 1). Organism_taxid: 11104. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Authors:

Z.P.Brown,I.S.Abaeva,S.De,C.U.T.Hellen,T.V.Pestova,J.Frank

Key ref:

Z.P.Brown et al. Molecular architecture of 40s translation initiation complexes on the hepatitis c virus ires.. Embo j, . PubMed id: 35822879

Date:

25-Nov-21 Release date: 27-Jul-22

Protein chain

G1TLT8  (G1TLT8_RABIT) -  Small ribosomal subunit protein uS2 from Oryctolagus cuniculus

Seq: 295 a.a.

Struc: 217 a.a.*

Protein chain

G1SS70  (G1SS70_RABIT) -  Small ribosomal subunit protein eS1 from Oryctolagus cuniculus

Seq: 264 a.a.

Struc: 213 a.a.

Protein chain

G1SV32  (G1SV32_RABIT) -  Large ribosomal subunit protein uL30 from Oryctolagus cuniculus

Seq: 208 a.a.

Struc: 221 a.a.*

Protein chain

G1TNM3  (G1TNM3_RABIT) -  Small ribosomal subunit protein uS3 from Oryctolagus cuniculus

Seq: 243 a.a.

Struc: 228 a.a.

Protein chain

G1TA47  (G1TA47_RABIT) -  40S ribosomal protein S4 from Oryctolagus cuniculus

Seq: 263 a.a.

Struc: 262 a.a.

Protein chain

G1TFM5  (G1TFM5_RABIT) -  Small ribosomal subunit protein uS7 from Oryctolagus cuniculus

Seq: 204 a.a.

Struc: 191 a.a.

Protein chain

A0A5K1UJS7  (A0A5K1UJS7_RABIT) -  40S ribosomal protein S6 from Oryctolagus cuniculus

Seq: 249 a.a.

Struc: 237 a.a.

Protein chain

G1SVB0  (G1SVB0_RABIT) -  Small ribosomal subunit protein eS7 from Oryctolagus cuniculus

Seq: 194 a.a.

Struc: 185 a.a.

Protein chain

G1TJW1  (G1TJW1_RABIT) -  Small ribosomal subunit protein eS8 from Oryctolagus cuniculus

Seq: 208 a.a.

Struc: 206 a.a.*

Protein chain

B7NZS8  (RS9_RABIT) -  Small ribosomal subunit protein uS4 from Oryctolagus cuniculus

Seq: 194 a.a.

Struc: 185 a.a.

Protein chain

G1TPV3  (G1TPV3_RABIT) -  Small ribosomal subunit protein eS10 from Oryctolagus cuniculus

Seq: 165 a.a.

Struc: 96 a.a.

Protein chain

G1TRM4  (G1TRM4_RABIT) -  Small ribosomal subunit protein uS17 from Oryctolagus cuniculus

Seq: 158 a.a.

Struc: 151 a.a.

Protein chain

G1SFR8  (G1SFR8_RABIT) -  Small ribosomal subunit protein eS12 from Oryctolagus cuniculus

Seq: 132 a.a.

Struc: 117 a.a.

Protein chain

G1SP51  (G1SP51_RABIT) -  Small ribosomal subunit protein uS15 from Oryctolagus cuniculus

Seq: 151 a.a.

Struc: 149 a.a.

Protein chain

G1T1F0  (G1T1F0_RABIT) -  Small ribosomal subunit protein uS11 from Oryctolagus cuniculus

Seq: 151 a.a.

Struc: 136 a.a.

Protein chain

G1U0Q2  (G1U0Q2_RABIT) -  Small ribosomal subunit protein uS19 from Oryctolagus cuniculus

Seq: 145 a.a.

Struc: 120 a.a.

Protein chain

G1SGX4  (G1SGX4_RABIT) -  Small ribosomal subunit protein uS9 from Oryctolagus cuniculus

Seq: 146 a.a.

Struc: 142 a.a.

Protein chain

G1TU13  (G1TU13_RABIT) -  Small ribosomal subunit protein eS17 from Oryctolagus cuniculus

Seq: 135 a.a.

Struc: 132 a.a.

Protein chain

G1TPG3  (G1TPG3_RABIT) -  Small ribosomal subunit protein uS13 from Oryctolagus cuniculus

Seq: 152 a.a.

Struc: 144 a.a.

Protein chain

G1TN62  (G1TN62_RABIT) -  Small ribosomal subunit protein eS19 from Oryctolagus cuniculus

Seq: 145 a.a.

Struc: 141 a.a.

Protein chain

G1SIZ2  (G1SIZ2_RABIT) -  Small ribosomal subunit protein uS10 from Oryctolagus cuniculus

Seq: 119 a.a.

Struc: 100 a.a.

Protein chain

G1TM82  (G1TM82_RABIT) -  Small ribosomal subunit protein eS21 from Oryctolagus cuniculus

Seq: 83 a.a.

Struc: 83 a.a.*

Protein chain

G1TG89  (G1TG89_RABIT) -  Small ribosomal subunit protein uS8 from Oryctolagus cuniculus

Seq: 130 a.a.

Struc: 129 a.a.

Protein chain

G1SZ47  (G1SZ47_RABIT) -  Small ribosomal subunit protein uS12 from Oryctolagus cuniculus

Seq: 143 a.a.

Struc: 141 a.a.

Protein chain

G1T3D8  (G1T3D8_RABIT) -  40S ribosomal protein S24 from Oryctolagus cuniculus

Seq: 131 a.a.

Struc: 124 a.a.

Protein chain

G1TDB3  (G1TDB3_RABIT) -  Small ribosomal subunit protein eS25 from Oryctolagus cuniculus

Seq: 124 a.a.

Struc: 77 a.a.

Protein chain

G1TFE8  (G1TFE8_RABIT) -  Small ribosomal subunit protein eS26 from Oryctolagus cuniculus

Seq: 115 a.a.

Struc: 101 a.a.*

Protein chain

G1TZ76  (G1TZ76_RABIT) -  Small ribosomal subunit protein eS27 from Oryctolagus cuniculus

Seq: 84 a.a.

Struc: 83 a.a.

Protein chain

G1TIB4  (G1TIB4_RABIT) -  Small ribosomal subunit protein eS28 from Oryctolagus cuniculus

Seq: 69 a.a.

Struc: 67 a.a.

Protein chain

G1U7M4  (G1U7M4_RABIT) -  Small ribosomal subunit protein uS14 from Oryctolagus cuniculus

Seq: 56 a.a.

Struc: 55 a.a.

Protein chain

G1T8A2  (G1T8A2_RABIT) -  Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein from Oryctolagus cuniculus

Seq: 133 a.a.

Struc: 57 a.a.

Protein chain

G1SK22  (G1SK22_RABIT) -  Ubiquitin-ribosomal protein eS31 fusion protein from Oryctolagus cuniculus

Seq: 156 a.a.

Struc: 68 a.a.

Protein chain

G1SJB4  (G1SJB4_RABIT) -  Small ribosomal subunit protein RACK1 from Oryctolagus cuniculus

Seq: 317 a.a.

Struc: 313 a.a.

Protein chain

A0A087WNH4  (RS32_RABIT) -  Small ribosomal subunit protein eS32 from Oryctolagus cuniculus

Seq: 25 a.a.

Struc: 25 a.a.

* PDB and UniProt seqs differ at 86 residue positions (black crosses)

DNA/RNA chains

U-A-C-C-U-G-G-U-U-G-A-U-C-C-U-G-C-C-A-G-U-A-G-C-A-U-A-U-G-C-U-U-G-U-C-U-C-A-A- ... 1680 bases

A-A-A-A-A-A-A-A-A-A-A-A 12 bases

C-C-U-G-U-G-A-G-G-A-A-C-U-A-C-U-G-U-C-U-U-C-A-C-G-C-A-G-A-A-A-G-C-G-U-C-U-A-G- 249 bases

Enzyme reactions

• Enzyme class: Chain E: E.C.4.2.99.18 - DNA-(apurinic or apyrimidinic site) lyase.
• Reaction: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)- 2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho- 2'-deoxyribonucleoside-DNA + H⁺