PDBsum entry 7s3h

Hydrolase/RNA/DNA

PDB id: 7s3h

Contents

Protein chain

1030 a.a.

DNA/RNA

PDB id:

7s3h

Name:

Hydrolase/RNA/DNA

Title:

Cas9:sgrna:dna (s. Pyogenes) with 0 rna:dna base pairs, open- protein/linear-DNA conformation

Structure:

Non-target DNA strand. Chain: n. Engineered: yes. Crispr-associated endonuclease cas9/csn1. Chain: p. Synonym: spcas9,spycas9. Engineered: yes. Mutation: yes. Single-guide RNA.

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Streptococcus pyogenes serotype m1. Organism_taxid: 301447. Gene: cas9, csn1, spy_1046. Expressed in: escherichia coli. Expression_system_taxid: 562. Streptococcus pyogenes.

Authors:

J.C.Cofsky,K.M.Soczek,G.J.Knott,E.Nogales,J.A.Doudna

Key ref:

J.C.Cofsky et al. Crispr-Cas9 bends and twists DNA to read its sequence. Nat struct mol biol, . PubMed id: 35422516

Date:

06-Sep-21 Release date: 20-Apr-22

Protein chain

Q99ZW2  (CAS9_STRP1) -  CRISPR-associated endonuclease Cas9/Csn1 from Streptococcus pyogenes serotype M1

Seq: 1368 a.a.

Struc: 1030 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-A-A-G-A-T-G-A-G-A-C-A-A-T-G-G-C-G-A-T-T-A-C 23 bases

A-A-C-U-U-G-A-A-A-A-A-G-U-G-G-C-A-C-C-G-A-G-U-C-G-G-U-G-C-U 30 bases

G-T-A-A-T-2YR-G-C-C-A-T-T-G-T-C-T-C-A-T-C-T-T-T 23 bases

Enzyme reactions

• Enzyme class: E.C.3.1.-.-