PDBsum entry 7rdf

Flavoprotein

PDB id: 7rdf

Contents

Protein chain

375 a.a.

Ligands

4R2-6FA

GOL ×3

PEG

Waters ×432

PDB id:

7rdf

Name:

Flavoprotein

Title:

Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f co-crystallized in the presence of d-arginine

Structure:

Fad-dependent catabolic d-arginine dehydrogenase daua. Chain: a. Synonym: d-arginine dehydrogenase,dadh,d-arginine utilization protein a,dau. Engineered: yes

Source:

Pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1). Organism_taxid: 208964. Strain: atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1. Atcc: 15692. Gene: daua, pa3863. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.29Å R-factor: 0.128 R-free: 0.157

Authors:

R.A.G.Reis,A.Iyer,A.Agniswamy,I.T.Weber,G.Gadda

Key ref:

A.Iyer et al. (2021). Discovery of a new flavin n5-Adduct in a tyrosine to phenylalanine variant of d-Arginine dehydrogenase.. Arch.Biochem.Biophys., 715, 09100. PubMed id: 34864048 DOI: 10.1016/J.ABB.2021.109100

Date:

09-Jul-21 Release date: 22-Dec-21

Protein chain

Q9HXE3  (DAUA_PSEAE) -  FAD-dependent catabolic D-arginine dehydrogenase DauA from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 375 a.a.

Struc: 375 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.4.99.6 - D-arginine dehydrogenase.
• Reaction: D-arginine + A + H2O = 5-guanidino-2-oxopentanoate + AH2 + NH4⁺
• Cofactor: FAD

FAD (Bound ligand (Het Group name = 4R2) matches with 85.48% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site