PDBsum entry 7rcd

DNA binding protein/DNA

PDB id: 7rcd

Contents

Protein chain

287 a.a.

DNA/RNA

Metals

_CA ×2

Waters ×11

PDB id:

7rcd

Name:

DNA binding protein/DNA

Title:

Second stage reengineered variant of i-onui targeting human pd1 gene with activity enhancing substitutions

Structure:

I-onui_e-hpd1-c. Chain: a. Engineered: yes. DNA (26-mer). Chain: b. Engineered: yes. DNA (26-mer). Chain: c. Engineered: yes

Source:

Synthetic construct. Organism_taxid: 32630. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 32630

Resolution:

2.45Å R-factor: 0.240 R-free: 0.277

Authors:

N.C.Ubilla-Rodriguez,R.A.Werther,B.L.Stoddard

Key ref:

R.A.Werther et al. Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-Editing meganuclease. To be published, .

Date:

07-Jul-21 Release date: 29-Jun-22

Protein chain

No UniProt id for this chain

Struc: 287 a.a.

DNA/RNA chains

G-G-G-G-G-C-A-T-G-C-A-G-A-T-C-C-C-A-C-A-G-G-C-G-C-G 26 bases

C-C-G-C-G-C-C-T-G-T-G-G-G-A-T-C-T-G-C-A-T-G-C-C-C-C 26 bases