PDBsum entry 7qw7

Transferase

PDB id: 7qw7

Contents

Protein chain

553 a.a.

DNA/RNA

Ligands

SAH

Waters ×47

PDB id:

7qw7

Name:

Transferase

Title:

Adenine-specific DNA methyltransferase m.Bseci complexed with adohcy and cognate fully methylated DNA duplex

Structure:

Modification methylase bseci. Chain: a. Synonym: m.Bseci,adenine-specific methyltransferase bseci. Engineered: yes. Fully methylated DNA duplex. Chain: z. Engineered: yes. Fully methylated DNA duplex. Chain: y.

Source:

Geobacillus stearothermophilus. Organism_taxid: 1422. Gene: bsecim. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 1422

Resolution:

2.60Å R-factor: 0.199 R-free: 0.217

Authors:

D.A.Mitsikas,K.Kouyianou,D.Kotsifaki,M.Providaki,V.Bouriotis, N.M.Glykos,M.Kokkinidis

Key ref:

D.A.Mitsikas et al. Structure of m.Bseci DNA methyltransferase from geoba stearothermophilus.. To be published, .

Date:

24-Jan-22 Release date: 25-Jan-23

Protein chain

P43423  (MTC1_GEOSE) -  Type II methyltransferase M.BseCI from Geobacillus stearothermophilus

Seq: 579 a.a.

Struc: 553 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-C-A-T-C-G-6MA-T-C-G 10 bases

C-G-A-T-C-G-6MA-T-G-C 10 bases

Enzyme reactions

• Enzyme class: E.C.2.1.1.72 - site-specific DNA-methyltransferase (adenine-specific).
• Reaction: a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N₆-methyl- 2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H⁺

2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = N(6)-methyl- 2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site