PDBsum entry 7py7

Transcription

PDB id: 7py7

Contents

Protein chains

228 a.a.

1341 a.a.

1335 a.a.

90 a.a.

123 a.a.

495 a.a.

DNA/RNA

Metals

_ZN ×2

_MG

PDB id:

7py7

Name:

Transcription

Title:

Cryoem structure of e.Coli RNA polymerase elongation complex bound to nusa and nusg (nusa and nusg elongation complex in more-swiveled conformation)

Structure:

Ntdna. Chain: n. Engineered: yes. Tdna. Chain: t. Engineered: yes. RNA. Chain: r. Engineered: yes.

Source:

Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: rpoa, pez, phs, sez, b3295, jw3257. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: rpob, z5560, ecs4910. Gene: rpoc, z5561, ecs4911. Gene: rpoz, b3649, jw3624.

Authors:

C.Zhu,X.Guo,A.Weixlbaumer

Key ref:

c.zhu et al. (2022). Transcription factors modulate RNA polymerase conformational equilibrium. Nat Commun, 13, 1546-1546. PubMed id: 35318334

Date:

09-Oct-21 Release date: 23-Mar-22

Protein chains

P0A7Z4  (RPOA_ECOLI) -  DNA-directed RNA polymerase subunit alpha from Escherichia coli (strain K12)

Seq: 329 a.a.

Struc: 228 a.a.

Protein chain

P0A8V2  (RPOB_ECOLI) -  DNA-directed RNA polymerase subunit beta from Escherichia coli (strain K12)

Seq: 1342 a.a.

Struc: 1341 a.a.

Protein chain

P0A8T7  (RPOC_ECOLI) -  DNA-directed RNA polymerase subunit beta' from Escherichia coli (strain K12)

Seq: 1407 a.a.

Struc: 1335 a.a.

Protein chain

P0A800  (RPOZ_ECOLI) -  DNA-directed RNA polymerase subunit omega from Escherichia coli (strain K12)

Seq: 91 a.a.

Struc: 90 a.a.

Protein chain

P0AFG0  (NUSG_ECOLI) -  Transcription termination/antitermination protein NusG from Escherichia coli (strain K12)

Seq: 181 a.a.

Struc: 123 a.a.

Protein chain

P0AFF6  (NUSA_ECOLI) -  Transcription termination/antitermination protein NusA from Escherichia coli (strain K12)

Seq: 495 a.a.

Struc: 495 a.a.

DNA/RNA chains

C-G-T-C-C-G-A-T-C-T-T-C-G-G-A-A-G-A-G-A-T-T-C-A-G-A-G 27 bases

C-T-C-T-G-A-A-T-C-T-C-T-T-C-C-G-A-C-G-C-G-C-C-G-C-G-G-G-A-C-G 31 bases

U-C-C-G-C-G-G-C-G-C-G 11 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Nat Commun 13:1546-1546 (2022)

PubMed id: 35318334

Transcription factors modulate RNA polymerase conformational equilibrium.

c.zhu, x.guo, a.weixlbaumer.

ABSTRACT

No abstract given.