PDBsum entry 7pev

DNA binding protein

PDB id: 7pev

Contents

Protein chains

97 a.a.

83 a.a.

109 a.a.

95 a.a.

DNA/RNA

PDB id:

7pev

Name:

DNA binding protein

Title:

Nucleosome stack of the 4x177 nucleosome array containing h1

Structure:

Histone h3.2. Chain: a, e, k, o. Synonym: histone h3/m,histone h3/o. Engineered: yes. Histone h4. Chain: b, f, l, p. Engineered: yes. Histone h2a type 1-b/e. Chain: c, g, m, q.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hist2h3a, hist2h3c, h3f2, h3fm, hist2h3d. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hist1h4a, h4/a, h4fa, hist1h4b, h4/i, h4fi, hist1h4c, h4/g, h4fg, hist1h4d, h4/b, h4fb, hist1h4e, h4/j, h4fj, hist1h4f, h4/c, h4fc, hist1h4h, h4/h, h4fh, hist1h4i, h4/m, h4fm, hist1h4j, h4/e,

Authors:

M.Dombrowski,P.Cramer

Key ref:

M.Dombrowski et al. (2022). Histone h1 binding to nucleosome arrays depends on li DNA length and trajectory.. Nat struct mol biol, 29, 493. PubMed id: 35581345

Date:

11-Aug-21 Release date: 03-Aug-22

Protein chains

Q71DI3  (H32_HUMAN) -  Histone H3.2 from Homo sapiens

Seq: 136 a.a.

Struc: 97 a.a.*

Protein chains

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 109 a.a.

Protein chains

O60814  (H2B1K_HUMAN) -  Histone H2B type 1-K from Homo sapiens

Seq: 126 a.a.

Struc: 95 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-G-C-A-C-T-G-G-A-A-C-A-G-G-A-T-G-T-A-T-A-T-A-T-G-T-G-A-C-A-C-G-T-G-C-C-T-G-G- ... 347 bases

C-C-C-G-G-A-T-C-C-C-C-T-G-G-A-G-A-A-T-C-C-C-G-G-T-G-C-C-G-A-G-G-C-C-G-C-T-C-A- ... 347 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G, H, K, L, M, N, O, P, Q, R: E.C.?