PDBsum entry 7pec

Signaling protein

PDB id: 7pec

Contents

Protein chains

2192 a.a.

317 a.a.

1052 a.a.

317 a.a.

Ligands

IHP

PDB id:

7pec

Name:

Signaling protein

Title:

Cryo-em structure of deptor bound to human mtor complex 1, dept-bound subset local refinement

Structure:

Serine/threonine-protein kinase mtor. Chain: a. Synonym: fk506-binding protein 12-rapamycin complex-associated protein 1,fkbp12-rapamycin complex-associated protein,mammalian target of rapamycin,mtor,mechanistic target of rapamycin,rapamycin and fkbp12 target 1,rapamycin target protein 1. Engineered: yes. Target of rapamycin complex subunit lst8. Chain: c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mtor, frap, frap1, frap2, raft1, rapt1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: mlst8, gbl, lst8. Gene: rptor, kiaa1303, raptor. Gene: deptor, depdc6.

Authors:

M.Waelchli,T.Maier

Key ref:

M.Walchli et al. (2021). Regulation of human mtor complexes by deptor.. Elife, 10, . PubMed id: 34519268

Date:

09-Aug-21 Release date: 08-Sep-21

Protein chain

P42345  (MTOR_HUMAN) -  Serine/threonine-protein kinase mTOR from Homo sapiens

Seq: 2549 a.a.

Struc: 2192 a.a.*

Protein chain

Q9BVC4  (LST8_HUMAN) -  Target of rapamycin complex subunit LST8 from Homo sapiens

Seq: 326 a.a.

Struc: 317 a.a.

Protein chain

Q8N122  (RPTOR_HUMAN) -  Regulatory-associated protein of mTOR from Homo sapiens

Seq: 1335 a.a.

Struc: 1052 a.a.

Protein chain

Q8TB45  (DPTOR_HUMAN) -  DEP domain-containing mTOR-interacting protein from Homo sapiens

Seq: 409 a.a.

Struc: 317 a.a.*

* PDB and UniProt seqs differ at 58 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺
• Enzyme class 2: Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site