PDBsum entry 7pcx

Hydrolase

PDB id: 7pcx

Contents

Protein chains

(+ 0 more) 293 a.a.

Ligands

OEH ×6

GOL ×7

Metals

_CL ×6

Waters ×1159

PDB id:

7pcx

Name:

Hydrolase

Title:

X-ray structure of the haloalkane dehalogenase halotag7-q165w labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate

Structure:

Haloalkane dehalogenase. Chain: a, b, c, d, e, f. Engineered: yes

Source:

Rhodococcus sp.. Organism_taxid: 1831. Gene: dhaa. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.40Å R-factor: 0.177 R-free: 0.198

Authors:

M.Tarnawski,M.Frei,J.Hiblot,K.Johnsson

Key ref:

M.Tarnawski et al. X-Ray structure of the haloalkane dehalogenase halotag7-Q165w labeled with a chloroalkane-Tetramethylrhodamine fluorophore substra. To be published, . PubMed id: 34916672

Date:

04-Aug-21 Release date: 15-Dec-21

Protein chains

P0A3G3  (DHAA_RHOSO) -  Haloalkane dehalogenase from Rhodococcus sp

Seq: 293 a.a.

Struc: 293 a.a.*

* PDB and UniProt seqs differ at 23 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.8.1.5 - haloalkane dehalogenase.
• Reaction: 1-haloalkane + H2O = a halide anion + a primary alcohol + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site