PDBsum entry 7oxh

Isomerase

PDB id: 7oxh

Contents

Protein chains

155 a.a.

11 a.a.

Ligands

UNK-UNK

PE4 ×3

PEG

Metals

_CL

_NI ×2

Waters ×75

PDB id:

7oxh

Name:

Isomerase

Title:

Ttslyd with pseudo-wild-type s2 peptide

Structure:

Peptidyl-prolyl cis-trans isomerase. Chain: a. Engineered: yes. 30s ribosomal protein s2. Chain: b. Synonym: small ribosomal subunit protein us2. Engineered: yes. Fragment of 30s ribosomal protein s2 peptide. Chain: e.

Source:

Thermus thermophilus (strain atcc 27634 / dsm 579 / hb8). Organism_taxid: 300852. Strain: atcc 27634 / dsm 579 / hb8. Gene: ttha0346. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Escherichia coli (strain k12).

Resolution:

1.70Å R-factor: 0.204 R-free: 0.238

Authors:

S.Pazicky,J.Lei,C.Loew

Key ref:

S.Pazicky et al. (2022). Impact of distant peptide substrate residues on enzym activity of slyd.. Cell mol life sci, 79, 138. PubMed id: 35184231

Date:

22-Jun-21 Release date: 16-Mar-22

Protein chain

Q5SLE7  (Q5SLE7_THET8) -  Peptidyl-prolyl cis-trans isomerase from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 149 a.a.

Struc: 155 a.a.

Protein chain

P0A7V0  (RS2_ECOLI) -  Small ribosomal subunit protein uS2 from Escherichia coli (strain K12)

Seq: 241 a.a.

Struc: 11 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.5.2.1.8 - peptidylprolyl isomerase.
• Reaction: [protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)
• Enzyme class 3: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site