PDBsum entry 7orm

Viral protein

PDB id: 7orm

Contents

Protein chain

2145 a.a.

DNA/RNA

Metals

_MG

_ZN

PDB id:

7orm

Name:

Viral protein

Title:

La crosse virus polymerase at transcription early-elongation stage

Structure:

RNA (5'-r(p Ap Cp Gp Ap Gp Up Gp Up Cp Gp Up Ap Cp C)-3'). Chain: h. Engineered: yes. Other_details: 5prime vrna of la crosse m segment. Nucleotides g2, u3, a9 and c10 were mutated into c2, g3, c9 and g10. RNA (5'-r(p Ap Ap Ap Gp Up Ap Cp Ap Cp Up Ap Cp U)-3'). Chain: t. Engineered: yes. RNA (5'-r(p Up Ap Up Ap Ap Up Ap Gp Up Ap Gp Up Gp Up A)-

Source:

Synthetic: yes. La crosse virus. Organism_taxid: 11577. Bunyavirus la crosse. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: hi 5

Authors:

B.Arragain,Q.Durieux Trouilleton,F.Baudin,S.Cusack,G.Schoehn,H.Malet

Key ref:

B.Arragain et al. Structural snapshots of la crosse virus polymerase re the mechanisms underlying peribunyaviridae replicatio transcription. Nat commun, . PubMed id: 35173159

Date:

06-Jun-21 Release date: 16-Feb-22

Protein chain

A5HC98  (L_BUNLC) -  RNA-directed RNA polymerase L from Bunyavirus La Crosse

Seq: 2263 a.a.

Struc: 2145 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-C-G-A-G-U-G-U-C-G-U-A-C-C 14 bases

A-G-U-A-C-A-C-U-A-C-U 11 bases

U-A-U-A-A-U-A-G-U-A-G-U-G-U-A 15 bases

Enzyme reactions

• Enzyme class 2: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.1.-.-

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site