PDBsum entry 7odh

Electron transport

PDB id: 7odh

Contents

Protein chains

598 a.a.

267 a.a.

Ligands

NFV

SF4 ×2

__O

F4S-__O

Metals

_MG

_CL ×5

Waters ×933

PDB id:

7odh

Name:

Electron transport

Title:

Crystal structure of the o2-tolerant mbh-p242c from ralstonia eutropha in its as-isolated state

Structure:

Uptake hydrogenase large subunit. Chain: l. Synonym: hydrogenlyase,membrane-bound hydrogenase large subunit. Engineered: yes. Uptake hydrogenase small subunit. Chain: s. Synonym: hydrogenlyase,membrane-bound hydrogenase small subunit. Engineered: yes. Mutation: yes

Source:

Cupriavidus necator (strain atcc 17699 / h16 / dsm 428 / stanier 337). Organism_taxid: 381666. Strain: atcc 17699 / h16 / dsm 428 / stanier 337. Atcc: 17699. Gene: hoxg, phg002. Expressed in: cupriavidus necator h16. Expression_system_taxid: 381666. Expression_system_atcc_number: 17699.

Resolution:

1.34Å R-factor: 0.113 R-free: 0.133

Authors:

A.Schmidt,J.Kalms,P.Scheerer

Key ref:

E.Siebert et al. Resonance raman spectroscopic analysis of the iron-Su cluster redox chain of the ralstonia eutropha membran [nife]-Hydrogenase. J raman spectrosc, .

Date:

29-Apr-21 Release date: 07-Jul-21

Protein chain

P31891  (MBHL_CUPNH) -  Uptake hydrogenase large subunit from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)

Seq: 618 a.a.

Struc: 598 a.a.

Protein chain

P31892  (MBHS_CUPNH) -  Uptake hydrogenase small subunit from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)

Seq: 360 a.a.

Struc: 269 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains L, S: E.C.1.12.99.6 - hydrogenase (acceptor).
• Reaction: H2 + A = AH2
• Cofactor: Iron-sulfur; Ni(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site