PDBsum entry 7ocs

Oxidoreductase

PDB id: 7ocs

Contents

Protein chains

682 a.a.

572 a.a.

Ligands

GOL ×2

EDO ×13

PGE ×2

PG4 ×2

BME ×2

SO4 ×10

44H ×4

EPE

ACT

Metals

_MG

_CL ×10

_NA ×2

Waters ×572

PDB id:

7ocs

Name:

Oxidoreductase

Title:

Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld-d16a from acinetobacter baumannii

Structure:

Had hydrolase, family ia, variant 3. Chain: a, b, c, d. Engineered: yes

Source:

Acinetobacter baumannii atcc 19606 = cip 70.34 = jcm 6841. Organism_taxid: 575584. Strain: atcc 19606 / dsm 30007 / cip 70.34 / jcm 6841 / nbrc 109757 / ncimb 12457 / nctc 12156 / 81. Atcc: 19606. Gene: hmpref0010_00722. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.30Å R-factor: 0.217 R-free: 0.247

Authors:

H.K.Tam,V.Mueller,K.M.Pos

Key ref:

H.K.Tam et al. (2022). Unidirectional mannitol synthesis of acinetobacter ba mtld is facilitated by the helix-Loop-Helix-Mediated formation.. Proc natl acad sci usa, 119, 94119. PubMed id: 35363566

Date:

28-Apr-21 Release date: 27-Apr-22

Protein chains

D0C7J2  (D0C7J2_ACIB2) -  HAD hydrolase, family IA, variant 3 from Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)

Seq: 715 a.a.

Struc: 682 a.a.*

Protein chain

D0C7J2  (D0C7J2_ACIB2) -  HAD hydrolase, family IA, variant 3 from Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)

Seq: 715 a.a.

Struc: 572 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)