PDBsum entry 7o7c

Fluorescent protein

PDB id: 7o7c

Contents

Protein chain

233 a.a.

Ligands

SO4 ×3

Waters ×275

PDB id:

7o7c

Name:

Fluorescent protein

Title:

Crystal structure of rsegfp2 mutant v151a in the non-fluorescent off- state determined by synchrotron radiation at 100k

Structure:

Green fluorescent protein. Chain: a. Engineered: yes. Mutation: yes

Source:

Aequorea victoria. Organism_taxid: 6100. Gene: gfp. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.55Å R-factor: 0.166 R-free: 0.196

Authors:

J.Woodhouse,V.Adam,K.Hadjidemetriou,J.-P.Colletier,M.Weik

Key ref:

j.woodhouse et al. (2022). Rational Control of Off-State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement. Chemphyschem, 0, e202200192. PubMed id: 35959919

Date:

13-Apr-21 Release date: 27-Jul-22

Protein chain

P42212  (GFP_AEQVI) -  Green fluorescent protein from Aequorea victoria

Seq: 238 a.a.

Struc: 233 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.?

Chemphyschem 0:e202200192 (2022)

PubMed id: 35959919

Rational Control of Off-State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement.

j.woodhouse, v.adam, k.hadjidemetriou, j.p.colletier, m.w.

ABSTRACT

No abstract given.