PDBsum entry 7nqw

Signaling protein

PDB id: 7nqw

Contents

Protein chain

346 a.a.

Ligands

SO4 ×2

DMS ×2

UNW

Waters ×423

PDB id:

7nqw

Name:

Signaling protein

Title:

Discovery of astx029, a clinical candidate which modulates the phosphorylation and catalytic activity of erk1/2

Structure:

Mitogen-activated protein kinase 1. Chain: a. Synonym: mapk 1,ert1,extracellular signal-regulated kinase 2,erk-2, map kinase isoform p42,p42-mapk,mitogen-activated protein kinase 2, mapk 2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mapk1, erk2, prkm1, prkm2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.78Å R-factor: 0.167 R-free: 0.206

Authors:

M.O'Reilly

Key ref:

T.D.Heightman et al. (2021). Discovery of astx029, A clinical candidate which modu the phosphorylation and catalytic activity of erk1/2.. J med chem, 64, 12286. PubMed id: 34387469

Date:

02-Mar-21 Release date: 06-Oct-21

Protein chain

P28482  (MK01_HUMAN) -  Mitogen-activated protein kinase 1 from Homo sapiens

Seq: 360 a.a.

Struc: 346 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.24 - mitogen-activated protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site