PDBsum entry 7nqf

Transferase

PDB id: 7nqf

Contents

Protein chains

218 a.a.

DNA/RNA

Metals

_CO ×4

Waters ×170

PDB id:

7nqf

Name:

Transferase

Title:

Prim-pol domain of crispr-associated prim-pol (capp) from marinitoga sp. 1137 with dsdna

Structure:

Tpr_region domain-containing protein. Chain: a, b. Engineered: yes. DNA(cgtgdg). Chain: c, d, e, f. Engineered: yes

Source:

Marinitoga sp. 1137. Organism_taxid: 1545835. Strain: dsm 14283 / jcm 11233 / ka3. Gene: marpi_0402. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

2.02Å R-factor: 0.214 R-free: 0.259

Authors:

A.W.H.Li,A.J.Doherty

Key ref:

A.W.H.Li et al. (2022). Molecular basis for the initiation of DNA primer synt. Nature, 605, . PubMed id: 35508653

Date:

01-Mar-21 Release date: 04-May-22

Protein chains

H2J4R1  (H2J4R1_MARPK) -  TOTE conflict system primase domain-containing protein from Marinitoga piezophila (strain DSM 14283 / JCM 11233 / KA3)

Seq: 546 a.a.

Struc: 218 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

DNA/RNA chains

C-G-T-G-C-G 6 bases

C-G-T-G-C-G 6 bases

C-G-T-G-C-G 6 bases

C-G-T-G-C-G 6 bases