PDBsum entry 7n6t

Hydrolase

PDB id: 7n6t

Contents

Protein chains

99 a.a.

Waters ×161

PDB id:

7n6t

Name:

Hydrolase

Title:

Crystal structure of inhibitor-free HIV-1 prs17 revertant mutant prs17 v48g

Structure:

Protease. Chain: a, b. Synonym: pr,retropepsin. Engineered: yes. Mutation: yes

Source:

Human immunodeficiency virus type 1 group m subtype b (isolate bru/lai). HIV-1. Organism_taxid: 11686. Strain: isolate bru/lai. Variant: prs17 v48g. Gene: gag-pol. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.32Å R-factor: 0.159 R-free: 0.186

Authors:

S.H.Burnaman,Y.-F.Wang,I.T.Weber

Key ref:

S.H.Burnaman et al. (2021). Revertant mutation v48g alters conformational dynamic highly drug resistant HIV protease prs17.. J.Mol.Graph.Model., 108, 08005. PubMed id: 34419931 DOI: 10.1016/J.JMGM.2021.108005

Date:

09-Jun-21 Release date: 08-Sep-21

Protein chains

P03367  (POL_HV1BR) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI)

Seq: 1447 a.a.

Struc: 99 a.a.*

* PDB and UniProt seqs differ at 19 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 4: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 5: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site