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PDBsum entry 7n2x
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Oxidoreductase
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PDB id
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7n2x
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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The crystal structure of an fmn-dependent nadh:quinone oxidoreductase, azor from escherichia coli
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Structure:
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Fmn-dependent nadh:quinone oxidoreductase. Chain: a. Synonym: azo-dye reductase,fmn-dependent nadh-azo compound oxidoreductase,fmn-dependent nadh-azoreductase. Engineered: yes
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Source:
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Escherichia coli o157:h7. Organism_taxid: 83334. Gene: azor, z2315, ecs2014. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008
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Resolution:
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1.70Å
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R-factor:
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0.167
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R-free:
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0.190
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Authors:
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A.J.Arcinas,E.Fedorov,L.Kelly,S.C.Almo,A.Ghosh
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Key ref:
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R.Hitchings
et al.
Uncovering a novel mechanism of enzyme activation in multimeric azoreductases.
To be published,
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Date:
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30-May-21
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Release date:
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31-Aug-22
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PROCHECK
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Headers
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References
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P41407
(AZOR_ECOLI) -
FMN-dependent NADH:quinone oxidoreductase from Escherichia coli (strain K12)
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Seq:
Struc:
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201 a.a.
201 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class:
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E.C.1.7.1.17
- FMN-dependent NADH-azoreductase.
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Reaction:
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N,N-dimethyl-1,4-phenylenediamine + anthranilate + 2 NAD+ = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+
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N,N-dimethyl-1,4-phenylenediamine
Bound ligand (Het Group name = )
matches with 45.45% similarity
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+
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anthranilate
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+
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2
×
NAD(+)
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=
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2-(4-dimethylaminophenyl)diazenylbenzoate
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+
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2
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NADH
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+
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2
×
H(+)
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Cofactor:
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FMN
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FMN
Bound ligand (Het Group name =
FMN)
corresponds exactly
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
