PDBsum entry 7mcs

DNA binding protein/DNA

PDB id: 7mcs

Contents

Protein chains

249 a.a.

402 a.a.

358 a.a.

DNA/RNA

Ligands

ANP ×6

Metals

_MG ×6

PDB id:

7mcs

Name:

DNA binding protein/DNA

Title:

Cryo-electron microscopy structure of tnsc(1-503)a225v bound to DNA

Structure:

Transposon tn7 transposition protein tnsc. Chain: a, b, c, d, e, f, g. Synonym: protein e. Engineered: yes. Mutation: yes. DNA (5'-d(p Cp Gp Cp Gp Cp Gp Cp Gp Cp Gp Cp Gp Cp Gp C)- 3'). Chain: h. Engineered: yes.

Source:

Escherichia coli. Organism_taxid: 562. Gene: tnsc. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Authors:

Y.Shen,J.Ortega,A.Guarne

Key ref:

Y.Shen et al. (2022). Structural basis for DNA targeting by the tn7 transpo. Nat struct mol biol, 29, . PubMed id: 35173349

Date:

02-Apr-21 Release date: 23-Feb-22

Protein chain

P05846  (TNSC_ECOLX) -  Transposon Tn7 transposition protein TnsC from Escherichia coli

Seq: 555 a.a.

Struc: 249 a.a.*

Protein chains

P05846  (TNSC_ECOLX) -  Transposon Tn7 transposition protein TnsC from Escherichia coli

Seq: 555 a.a.

Struc: 402 a.a.*

Protein chain

P05846  (TNSC_ECOLX) -  Transposon Tn7 transposition protein TnsC from Escherichia coli

Seq: 555 a.a.

Struc: 358 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

C-G-C-G-C-G-C-G-C-G-C-G-C-G-C 15 bases

G-C-G-C-G-C-G-C-G-C-G-C-G-C-G 15 bases