PDBsum entry 7lyc

Structural protein/DNA

PDB id: 7lyc

Contents

Protein chains

99 a.a.

88 a.a.

95 a.a.

83 a.a.

80 a.a.

335 a.a.

108 a.a.

DNA/RNA

PDB id:

7lyc

Name:

Structural protein/DNA

Title:

Cryo-em structure of the human nucleosome core particle ubiquitylated at histone h2a lys13 and lys15 in complex with bard1 (residues 415- 777)

Structure:

Histone h3.1. Chain: a, e. Synonym: histone h3/a,histone h3/b,histone h3/c,histone h3/d,histone h3/f,histone h3/h,histone h3/i,histone h3/j,histone h3/k,histone h3/l. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: h3c1, h3fa, hist1h3a, h3c2, h3fl, hist1h3b, h3c3, h3fc hist1h3c, h3c4, h3fb, hist1h3d, h3c6, h3fd, hist1h3e, h3c7, h3fi, hist1h3f, h3c8, h3fh, hist1h3g, h3c10, h3fk, hist1h3h, h3c11, h3ff, hist1h3i, h3c12, h3fj, hist1h3j. Expressed in: escherichia coli. Expression_system_taxid: 562.

Authors:

Q.Hu,M.V.Botuyan,D.Zhao,D.Cui,E.Mer,G.Mer

Key ref:

Q.Hu et al. Cryo-Em structure of the human nucleosome core partic ubiquitylated at histone h2a lys13 and lys15 in compl bard1 (residues 415-777). To be published, . PubMed id: 34321665

Date:

06-Mar-21 Release date: 16-Jun-21

Protein chains

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 99 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 88 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 95 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

Protein chain

P0CG47  (UBB_HUMAN) -  Polyubiquitin-B from Homo sapiens

Seq: 229 a.a.

Struc: 80 a.a.

Protein chain

Q99728  (BARD1_HUMAN) -  BRCA1-associated RING domain protein 1 from Homo sapiens

Seq: 777 a.a.

Struc: 335 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 108 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-T-C-G-A-G-A-A-T-C-C-C-G-G-T-G-C-C-G-A-G-G-C-C-G-C-T-C-A-A-T-T-G-G-T-C-G-T-A- 147 bases

T-C-G-G-A-T-G-T-A-T-A-T-A-T-C-T-G-A-C-A-C-G-T-G-C-C-T-G-G-A-G-A-C-T-A-G-G-G-A- 146 bases

Enzyme reactions

• Enzyme class: Chain N: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine