PDBsum entry 7lp2

Ligase

PDB id: 7lp2

Contents

Protein chains

33 a.a.

11 a.a.

12 a.a.

37 a.a.

15 a.a.

Ligands

GOL ×2

SO4 ×4

Waters ×107

PDB id:

7lp2

Name:

Ligase

Title:

Structure of nedd4l ww3 domain

Structure:

E3 ubiquitin-protein ligase. Chain: a, c, e. Engineered: yes. Angiomotin. Chain: b, d, f. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: nedd4l. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.88Å R-factor: 0.190 R-free: 0.228

Authors:

A.Alian,S.L.Alam,T.Thompson,L.Rheinemann,W.I.Sundquist

Key ref:

L.Rheinemann et al. (2021). Interactions between amot ppxy motifs and nedd4l ww d function in HIV-1 release.. J.Biol.Chem., 297, 00975. PubMed id: 34284061 DOI: 10.1016/J.JBC.2021.100975

Date:

11-Feb-21 Release date: 28-Jul-21

Protein chains

Q96PU5  (NED4L_HUMAN) -  E3 ubiquitin-protein ligase NEDD4-like from Homo sapiens

Seq: 975 a.a.

Struc: 33 a.a.

Protein chain

Q4VCS5  (AMOT_HUMAN) -  Angiomotin from Homo sapiens

Seq: 1084 a.a.

Struc: 11 a.a.

Protein chain

Q4VCS5  (AMOT_HUMAN) -  Angiomotin from Homo sapiens

Seq: 1084 a.a.

Struc: 12 a.a.

Protein chain

Q96PU5  (NED4L_HUMAN) -  E3 ubiquitin-protein ligase NEDD4-like from Homo sapiens

Seq: 975 a.a.

Struc: 37 a.a.*

Protein chain

Q4VCS5  (AMOT_HUMAN) -  Angiomotin from Homo sapiens

Seq: 1084 a.a.

Struc: 15 a.a.

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, C, E: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine