PDBsum entry 7lp1

Ligase

PDB id: 7lp1

Contents

Protein chain

39 a.a.

Ligands

NO3 ×3

GOL ×2

Waters ×43

PDB id:

7lp1

Name:

Ligase

Title:

Structure of nedd4l ww3 domain

Structure:

E3 ubiquitin-protein ligase nedd4-like. Chain: a. Fragment: ww 3 domain, residues 494-532. Synonym: hect-type e3 ubiquitin transferase ned4l,nedd4.2,nedd4-2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: nedd4l, kiaa0439, nedl3. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

1.35Å R-factor: 0.187 R-free: 0.214

Authors:

A.Alian,S.L.Alam,T.Thompson,L.Rheinemann,W.I.Sundquist

Key ref:

L.Rheinemann et al. (2021). Interactions between amot ppxy motifs and nedd4l ww d function in HIV-1 release.. J.Biol.Chem., 297, 00975. PubMed id: 34284061 DOI: 10.1016/J.JBC.2021.100975

Date:

11-Feb-21 Release date: 28-Jul-21

Protein chain

Q96PU5  (NED4L_HUMAN) -  E3 ubiquitin-protein ligase NEDD4-like from Homo sapiens

Seq: 975 a.a.

Struc: 39 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine