PDBsum entry 7ldc

Transferase

PDB id: 7ldc

Contents

Protein chains

390 a.a.

Ligands

SO4 ×10

COA ×4

Waters ×535

PDB id:

7ldc

Name:

Transferase

Title:

Zoogloea ramigera biosynthetic thiolase q183y/y218e mutant

Structure:

Acetyl-coa acetyltransferase. Chain: a, b, c, d. Synonym: acetoacetyl-coa thiolase,beta-ketothiolase. Engineered: yes. Mutation: yes

Source:

Zoogloea ramigera. Organism_taxid: 350. Gene: phaa, phba. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.50Å R-factor: 0.221 R-free: 0.260

Authors:

A.C.Marshall,J.B.Bruning

Key ref:

A.C.Marshall and j.b.bruning (2021). Engineering potassium activation into biosynthetic th. Biochem j, 478, 3047. PubMed id: 34338286

Date:

13-Jan-21 Release date: 01-Sep-21

Protein chains

P07097  (THIL_SHIZO) -  Acetyl-CoA acetyltransferase from Shinella zoogloeoides

Seq: 392 a.a.

Struc: 390 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.1.9 - acetyl-CoA C-acetyltransferase.
• Pathway: Mevalonate Biosynthesis
• Reaction: 2 acetyl-CoA = acetoacetyl-CoA + CoA

2 × acetyl-CoA = acetoacetyl-CoA + CoA (Bound ligand (Het Group name = COA) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site