PDBsum entry 7l9h

Hydrolase

PDB id: 7l9h

Contents

Protein chains

336 a.a.

Ligands

AR6 ×4

Metals

_MG ×4

Waters ×909

PDB id:

7l9h

Name:

Hydrolase

Title:

Crystal structure of human arh3-d77a bound to magnesium and adp-ribose

Structure:

Adp-ribose glycohydrolase arh3. Chain: c, d, a, b. Synonym: adp-ribosylhydrolase 3,o-acetyl-adp-ribose deacetylase arh3, poly(adp-ribose) glycohydrolase arh3,[protein adp-ribosylarginine] hydrolase-like protein 2,[protein adp-ribosylserine] hydrolase. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: adprs, adprhl2, arh3. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.85Å R-factor: 0.183 R-free: 0.227

Authors:

Y.Pourfarjam,I.Kurinov,J.Moss,I.K.Kim

Key ref:

y.pourfarjam et al. (2021). Structural and biochemical analysis of human ADP-ribosyl-acceptor hydrolase 3 (ARH3) reveals the basis of metal selectivity and different roles for the two Mg ions. J.Biol.Chem., 0, 100692-100692. PubMed id: 33894202

Date:

04-Jan-21 Release date: 28-Apr-21

Protein chains

Q9NX46  (ARHL2_HUMAN) -  ADP-ribosylhydrolase ARH3 from Homo sapiens

Seq: 363 a.a.

Struc: 336 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.2.1.143 - poly(ADP-ribose) glycohydrolase.
• Pathway: Poly(ADP-ribose) Glycohydrolase
• Reaction: [(1''->2')-ADP-alpha-D-ribose](n) + H2O = [(1''->2')-ADP-alpha-D- ribose](n-1) + ADP-D-ribose

[(1''->2')-ADP-alpha-D-ribose](n) + H2O = [(1''->2')-ADP-alpha-D- ribose](n-1) + ADP-D-ribose (Bound ligand (Het Group name = AR6) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

J.Biol.Chem. 0:100692-100692 (2021)

PubMed id: 33894202

Structural and biochemical analysis of human ADP-ribosyl-acceptor hydrolase 3 (ARH3) reveals the basis of metal selectivity and different roles for the two Mg ions.

y.pourfarjam, z.ma, i.kurinov, j.moss, i.k.kim.

ABSTRACT

No abstract given.