PDBsum entry 7kcl

Chaperone

PDB id: 7kcl

Contents

Protein chains

612 a.a.

23 a.a.

Ligands

ANP ×2

Metals

__K ×2

_MG ×2

PDB id:

7kcl

Name:

Chaperone

Title:

Full-length human mitochondrial hsp90 (trap1) in complex with sdhb in the presence of amp-pnp

Structure:

Heat shock protein 75 kda, mitochondrial. Chain: a, b. Synonym: hsp 75,tnfr-associated protein 1,tumor necrosis factor type 1 receptor-associated protein,trap-1. Engineered: yes. Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial. Chain: c. Fragment: unp residues 29-160.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: trap1, hsp75. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sdhb, sdh, sdh1. Expression_system_taxid: 562

Authors:

Y.X.Liu,D.A.Agard

Key ref:

Y.X.Liu et al. Cryo-Em reveals the dynamic interplay between mitocho hsp90 and sdhb folding intermediates. To be published, .

Date:

06-Oct-20 Release date: 13-Oct-21

Protein chains

Q12931  (TRAP1_HUMAN) -  Heat shock protein 75 kDa, mitochondrial from Homo sapiens

Seq: 704 a.a.

Struc: 612 a.a.*

Protein chain

P21912  (SDHB_HUMAN) -  Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial from Homo sapiens

Seq: 280 a.a.

Struc: 23 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chain C: E.C.1.1.5.- - ?????
• Enzyme class 2: Chain C: E.C.1.3.5.1 - succinate dehydrogenase.

Pathway:

• Reaction: a quinone + succinate = fumarate + a quinol
• Cofactor: FAD; Iron-sulfur

FAD (Bound ligand (Het Group name = ANP) matches with 44.83% similarity) Iron-sulfur

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site