PDBsum entry 7flc

Splicing

PDB id: 7flc

Contents

Protein chains

237 a.a.

300 a.a.

Ligands

VPW

Waters ×36

PDB id:

7flc

Name:

Splicing

Title:

Pandda analysis group deposition -- aar2/rnaseh in complex with fragment p05b08 from the f2x-universal library

Structure:

Pre-mRNA-splicing factor 8. Chain: a. Fragment: unp residues 1836-2090. Engineered: yes. A1 cistron-splicing factor aar2. Chain: b. Engineered: yes

Source:

Saccharomyces cerevisiae s288c. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: prp8, dbf3, dna39, rna8, slt21, usa2, yhr165c. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: aar2, ybl074c, ybl06.06, ybl0611. Expression_system_taxid: 562

Resolution:

1.95Å R-factor: 0.260 R-free: 0.318

Authors:

T.Barthel,J.Wollenhaupt,G.M.A.Lima,M.C.Wahl,M.S.Weiss

Key ref:

T.Barthel et al. (2022). Large-Scale crystallographic fragment screening exped compound optimization and identifies putative protein-Protein interaction sites.. J med chem, 65, 14630. PubMed id: 36260741

Date:

26-Aug-22 Release date: 02-Nov-22

Protein chain

P33334  (PRP8_YEAST) -  Pre-mRNA-splicing factor 8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 2413 a.a.

Struc: 237 a.a.*

Protein chain

P32357  (AAR2_YEAST) -  A1 cistron-splicing factor AAR2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 355 a.a.

Struc: 300 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)