PDBsum entry 7f8d

Oxidoreductase

PDB id: 7f8d

Contents

Protein chains

313 a.a.

Ligands

NAD ×4

Waters ×204

PDB id:

7f8d

Name:

Oxidoreductase

Title:

Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) g218y mutant

Structure:

Malate dehydrogenase. Chain: a, b, c, d. Engineered: yes. Mutation: yes

Source:

Geobacillus stearothermophilus. Bacillus stearothermophilus. Organism_taxid: 1422. Gene: mdh. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.40Å R-factor: 0.180 R-free: 0.258

Authors:

Y.Shimozawa,T.Himiyama,T.Nakamura,Y.Nishiya

Key ref:

Y.Shimozawa et al. (2021). Increasing loop flexibility affords low-Temperature adaptation of a moderate thermophilic malate dehydrog from geobacillus stearothermophilus.. Protein eng des sel, 34, . PubMed id: 34850194

Date:

02-Jul-21 Release date: 23-Feb-22

Protein chains

A0A143T1U9  (A0A143T1U9_GEOSE) -  Malate dehydrogenase from Geobacillus stearothermophilus

Seq: 312 a.a.

Struc: 313 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.1.1.37 - malate dehydrogenase.
• Pathway: Citric acid cycle
• Reaction: (S)-malate + NAD⁺ = oxaloacetate + NADH + H⁺

(S)-malate + NAD(+) (Bound ligand (Het Group name = NAD) corresponds exactly) = oxaloacetate + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site