PDBsum entry 7f4c

Oxidoreductase

PDB id: 7f4c

Contents

Protein chains

300 a.a.

Ligands

NAP ×2

BU1 ×2

Waters ×238

PDB id:

7f4c

Name:

Oxidoreductase

Title:

The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii.

Structure:

Homoserine dehydrogenase. Chain: a, b. Engineered: yes

Source:

Sulfurisphaera tokodaii (strain dsm 16993 / jcm 10545 / nbrc 100140 / 7). Sulfolobus tokodaii. Organism_taxid: 273063. Strain: dsm 16993 / jcm 10545 / nbrc 100140 / 7. Gene: hom, st1519, stk_15190. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.213 R-free: 0.257

Authors:

K.Ogata,R.Kaneko,T.Kubota,K.Watanabe,E.Kurihara,T.Oshima,K.Yoshimune, M.Goto

Key ref:

T.Kubota et al. Conformational changes in the catalytic region are responsible to heat-Induced activation of hyperthermo homoserine dehydrogenase. To be published, . PubMed id: 35835834

Date:

18-Jun-21 Release date: 22-Jun-22

Protein chains

F9VNG5  (F9VNG5_SULTO) -  Homoserine dehydrogenase from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

Seq: 304 a.a.

Struc: 300 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.3 - homoserine dehydrogenase.
• Pathway: Threonine Biosynthesis
• Reaction:
  1. L-homoserine + NAD⁺ = L-aspartate 4-semialdehyde + NADH + H⁺
  2. L-homoserine + NADP⁺ = L-aspartate 4-semialdehyde + NADPH + H⁺

L-homoserine (Bound ligand (Het Group name = BU1) matches with 75.00% similarity) + NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) = L-aspartate 4-semialdehyde + NADH + H(+)

L-homoserine (Bound ligand (Het Group name = BU1) matches with 75.00% similarity) + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = L-aspartate 4-semialdehyde + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site