PDBsum entry 7ewh

Oxidoreductase

PDB id

7ewh

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Contents

			Protein chains

					302 a.a.

			Ligands

					HMT ×2

			Waters ×24

PDB id:

7ewh

Links

Name:

Oxidoreductase

Title:

Crystal structure of human phgdh in complex with homoharringtonine

Structure:

D-3-phosphoglycerate dehydrogenase. Chain: a, b. Synonym: 3-pgdh,2-oxoglutarate reductase,malate dehydrogenase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: phgdh, pgdh3. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.99Å

R-factor:

0.248

R-free:

0.348

Authors:

C.H.Hsieh,Y.S.Cheng,Y.S.Lee,H.C.Huang,H.F.Juan

Key ref:

C.H.Hsieh et al. Crystal structure of human phgdh in complex with homoharringtonine. To be published, . PubMed id: 37673018

Date:

25-May-21

Release date:

07-Dec-22

PROCHECK

	Headers

	References

Protein chains

O43175 (SERA_HUMAN) - D-3-phosphoglycerate dehydrogenase from Homo sapiens

Seq: Struc:

Seq: Struc:					533 a.a. 302 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class 2:

E.C.1.1.1.37 - malate dehydrogenase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Citric acid cycle

Reaction:

(S)-malate + NAD⁺ = oxaloacetate + NADH + H⁺

(S)-malate	+	NAD(+)	=	oxaloacetate	+	NADH	+	H(+)

Enzyme class 3:

E.C.1.1.1.399 - 2-oxoglutarate reductase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

(R)-2-hydroxyglutarate + NAD⁺ = 2-oxoglutarate + NADH + H⁺

(R)-2-hydroxyglutarate	+	NAD(+)	=	2-oxoglutarate	+	NADH	+	H(+)

Enzyme class 4:

E.C.1.1.1.95 - phosphoglycerate dehydrogenase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

(2R)-3-phosphoglycerate + NAD⁺ = 3-phosphooxypyruvate + NADH + H⁺

(2R)-3-phosphoglycerate	+	NAD(+)	=	3-phosphooxypyruvate	+	NADH	+	H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site