PDBsum entry 7elm

Immune system/RNA

PDB id: 7elm

Contents

Protein chains

417 a.a.

305 a.a.

292 a.a.

(+ 4 more) 334 a.a.

187 a.a.

227 a.a.

DNA/RNA

PDB id:

7elm

Name:

Immune system/RNA

Title:

Structure of csy-acrif24

Structure:

Type i-f crispr-associated protein csy1. Chain: a, k. Engineered: yes. Crispr type i-f/ypest-associated protein csy2. Chain: b, l. Synonym: type i-f crispr-associated protein csy2. Engineered: yes. Crispr-associated protein csy3. Chain: c, d, e, f, g, h, m, n, o, p, q, r.

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Gene: csy1, alp65_00954, ipc1505_30690. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: csy2, alp65_00953, eqh76_13810, nctc13437_01526, pacl_0128. Gene: ipc1505_30680, ipc36_28835. Expression_system_taxid: 562

Authors:

L.Zhang,Y.Feng

Key ref:

L.Yang et al. Insights into the inhibition of type i-F crispr-Cas s by a multifunctional anti-Crispr protein acrif24.. Nat commun, . PubMed id: 35411005

Date:

12-Apr-21 Release date: 20-Apr-22

Protein chains

A0A3A8DDU9  (A0A3A8DDU9_PSEAI) -  Uncharacterized protein from Pseudomonas aeruginosa

Seq: 434 a.a.

Struc: 417 a.a.

Protein chains

Q02MM0  (CSY2_PSEAB) -  CRISPR-associated protein Csy2 from Pseudomonas aeruginosa (strain UCBPP-PA14)

Seq: 327 a.a.

Struc: 305 a.a.

Protein chains

Q02MM1  (CSY3_PSEAB) -  CRISPR-associated protein Csy3 from Pseudomonas aeruginosa (strain UCBPP-PA14)

Seq: 342 a.a.

Struc: 292 a.a.*

Protein chains

Q02MM1  (CSY3_PSEAB) -  CRISPR-associated protein Csy3 from Pseudomonas aeruginosa (strain UCBPP-PA14)

Seq: 342 a.a.

Struc: 334 a.a.*

Protein chains

Q02MM2  (CAS6_PSEAB) -  CRISPR-associated endonuclease Cas6/Csy4 from Pseudomonas aeruginosa (strain UCBPP-PA14)

Seq: 187 a.a.

Struc: 187 a.a.*

Protein chains

No UniProt id for this chain

Struc: 227 a.a.

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DNA/RNA chains

C-U-A-A-G-A-A-A-U-U-C-A-C-G-G-C-G-G-G-C-U-U-G-A-U-G-U-C-C-G-C-G-U-C-U-A-C-C-U- 60 bases

C-U-A-A-G-A-A-A-U-U-C-A-C-G-G-C-G-G-G-C-U-U-G-A-U-G-U-C-C-G-C-G-U-C-U-A-C-C-U- 60 bases

Enzyme reactions

• Enzyme class: Chains I, S: E.C.3.1.-.-