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PDBsum entry 7ekd

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protein ligands links
Biosynthetic protein PDB id
7ekd

 

 

 

 

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Contents
Protein chain
343 a.a.
Ligands
PO4 ×4
AKG
MPD
J5X
Waters ×307
PDB id:
7ekd
Name: Biosynthetic protein
Title: Crystal structure of gibberellin 3-oxidase 2 (ga3ox2) in rice
Structure: Gibberellin 3-beta-dioxygenase 2. Chain: a. Synonym: ga 3-oxidase 2,osga3ox2,gibberellin 3 beta-hydroxylase 2, protein dwarf18. Engineered: yes
Source: Oryza sativa subsp. Japonica. Rice. Organism_taxid: 39947. Gene: ga3ox2, d18, os01g0177400, loc_os01g08220, osj_00594, p0013f10.29. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
1.90Å     R-factor:   0.166     R-free:   0.212
Authors: S.Takehara,K.Kawai,B.Mikami,M.Ueguchi-Tanaka
Key ref: K.Kawai et al. (2022). Evolutionary alterations in gene expression and enzym activities of gibberellin 3-Oxidase 1 in oryza.. Commun biol, 5, 67. PubMed id: 35046494
Date:
05-Apr-21     Release date:   16-Feb-22    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9FU53  (G3OX2_ORYSJ) -  Gibberellin 3-beta-dioxygenase 2 from Oryza sativa subsp. japonica
Seq:
Struc:
373 a.a.
343 a.a.
Key:    PfamA domain  Secondary structure

 Enzyme reactions 
   Enzyme class: E.C.1.14.11.15  - gibberellin 3beta-dioxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Diterpenoid biosynthesis
      Reaction: gibberellin A20 + 2-oxoglutarate + O2 = gibberellin A1 + succinate + CO2
gibberellin A20
Bound ligand (Het Group name = AKG)
corresponds exactly
+
2-oxoglutarate
Bound ligand (Het Group name = J5X)
matches with 95.83% similarity
+ O2
= gibberellin A1
+ succinate
+ CO2
      Cofactor: Fe(2+); L-ascorbate
Fe(2+)
L-ascorbate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 

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