PDBsum entry 7e1v

Oxidoreductase

PDB id: 7e1v

Contents

Protein chains

276 a.a.

552 a.a.

186 a.a.

139 a.a.

67 a.a.

145 a.a.

76 a.a.

524 a.a.

378 a.a.

213 a.a.

Ligands

CDL ×17

PLM ×4

HEA ×4

9Y0 ×2

HEM ×4

MQ9 ×10

FES ×2

9YF ×4

HEC ×4

Metals

_CU ×8

PDB id:

7e1v

Name:

Oxidoreductase

Title:

Cryo-em structure of apo hybrid respiratory supercomplex consisting of mycobacterium tuberculosis complexiii and mycobacterium smegmatis complexiv

Structure:

CytochromE C oxidase subunit 2. Chain: e, q. Synonym: cytochromE C oxidase subunit ii ctac. CytochromE C oxidase subunit 1. Chain: f, r. CytochromE C oxidase subunit 3. Chain: g, s. Synonym: cytochromE C oxidase subunit 3 ctae. CytochromE C oxidase polypeptide 4.

Source:

Mycobacterium smegmatis mc2 51. Organism_taxid: 1445611. Mycolicibacterium smegmatis mc2 51. Mycobacterium tuberculosis h37rv. Organism_taxid: 83332. Strain: h37rv. Gene: qcrb, rv2196, mtcy190.07. Expressed in: mycolicibacterium smegmatis mc2 155. Expression_system_taxid: 246196.

Authors:

S.Zhou,W.Wang,Y.Gao,H.Gong,Z.Rao

Key ref:

S.Zhou et al. Cryo-Em structure of apo hybrid respiratory supercomp consisting of mycobacterium tuberculosis complexiii a mycobacterium smegmatis complexiv. To be published, . PubMed id: 34819223

Date:

03-Feb-21 Release date: 13-Oct-21

Protein chains

A0R057  (A0R057_MYCS2) -  cytochrome-c oxidase from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 341 a.a.

Struc: 276 a.a.

Protein chains

No UniProt id for this chain

Struc: 552 a.a.

Protein chains

A0R049  (A0R049_MYCS2) -  Probable cytochrome c oxidase subunit 3 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 203 a.a.

Struc: 186 a.a.

Protein chains

A0R056  (A0R056_MYCS2) -  Cytochrome c oxidase polypeptide 4 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 139 a.a.

Struc: 139 a.a.

Protein chains

A0R1B6  (A0R1B6_MYCS2) -  Uncharacterized protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 79 a.a.

Struc: 67 a.a.

Protein chains

A0R1B5  (Y4692_MYCS2) -  Uncharacterized protein MSMEG_4692/MSMEI_4575 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 157 a.a.

Struc: 145 a.a.

Protein chains

A0QVH4  (A0QVH4_MYCS2) -  Transmembrane protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 84 a.a.

Struc: 76 a.a.

Protein chains

P9WP37  (QCRB_MYCTU) -  Cytochrome bc1 complex cytochrome b subunit from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 549 a.a.

Struc: 524 a.a.

Protein chains

P9WH23  (QCRA_MYCTU) -  Cytochrome bc1 complex Rieske iron-sulfur subunit from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 429 a.a.

Struc: 378 a.a.

Protein chains

P9WP35  (QCRC_MYCTU) -  Cytochrome bc1 complex cytochrome c subunit from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 280 a.a.

Struc: 213 a.a.

Enzyme reactions

• Enzyme class 1: Chains N, O, B, C: E.C.7.1.1.8 - quinol--cytochrome-c reductase.
• Reaction: a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)- [cytochrome c](out) + 2 H(+)(out)

quinol + 2 × Fe(III)-[cytochrome c](out) = quinone (Bound ligand (Het Group name = PLM) matches with 58.33% similarity) + 2 × Fe(II)- [cytochrome c](out) + 2 × H(+)(out)

• Enzyme class 2: Chains E, G, H, Q, S, T: E.C.7.1.1.9 - cytochrome-c oxidase.
• Reaction: 4 Fe(II)-[cytochrome c] + O2 + 8 H⁺(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H⁺(out)
• Cofactor: Cu cation

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site