PDBsum entry 7ddk

Membrane protein

PDB id: 7ddk

Contents

Protein chains

996 a.a.

291 a.a.

32 a.a.

Ligands

NAG-NAG ×4

PCW ×13

E4R ×2

CLR ×6

NAG ×2

Metals

_MG ×4

_NA ×2

Waters ×10

PDB id:

7ddk

Name:

Membrane protein

Title:

Crystal structures of na+,k+-atpase in complex with rostafuroxin

Structure:

Sodium/potassium-transporting atpase subunit alpha-1. Chain: a, c. Synonym: na(+)/k(+) atpase alpha-1 subunit,sodium pump subunit alpha- 1. Sodium/potassium-transporting atpase subunit beta-1. Chain: b, d. Synonym: sodium/potassium-dependent atpase subunit beta-1. Fxyd domain-containing ion transport regulator. Chain: g, e.

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Organism_taxid: 9823

Resolution:

3.50Å R-factor: 0.222 R-free: 0.269

Authors:

H.Ogawa,F.Cornelius,R.Kanai,K.Motoyama,B.Vilsen,C.Toyoshima

Key ref:

R.Kanai et al. (2021). Binding of cardiotonic steroids to na + ,K + -Atpase e2p state.. Proc natl acad sci usa, 118, . PubMed id: 33318128

Date:

29-Oct-20 Release date: 27-Jan-21

Supersedes:

6kq0

Protein chains

P05024  (AT1A1_PIG) -  Sodium/potassium-transporting ATPase subunit alpha-1 from Sus scrofa

Seq: 1021 a.a.

Struc: 996 a.a.*

Protein chains

P05027  (AT1B1_PIG) -  Sodium/potassium-transporting ATPase subunit beta-1 from Sus scrofa

Seq: 303 a.a.

Struc: 291 a.a.

Protein chains

Q58K79  (Q58K79_PIG) -  FXYD domain-containing ion transport regulator from Sus scrofa

Seq: 65 a.a.

Struc: 32 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, C: E.C.7.2.2.13 - Na(+)/K(+)-exchanging ATPase.
• Reaction: K⁺(out) + Na⁺(in) + ATP + H2O = K⁺(in) + Na(+)(out) + ADP + phosphate + H(+)

K(+)(out) + Na(+)(in) + ATP + H2O = K(+)(in) + Na(+)(out) + ADP (Bound ligand (Het Group name = NAG) matches with 41.38% similarity) + phosphate + H(+)

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site