PDBsum entry 7coh

Oxidoreductase

PDB id: 7coh

Contents

Protein chains

513 a.a.

227 a.a.

258 a.a.

143 a.a.

102 a.a.

91 a.a.

72 a.a.

75 a.a.

70 a.a.

56 a.a.

49 a.a.

44 a.a.

40 a.a.

Ligands

HEA ×4

CDL ×6

CHD ×6

DMU ×37

PER ×2

LFA ×24

EDO ×29

CUA ×2

DMU-LFA ×2

UNX ×2

PEK ×2

PGV

DMU-DMU-DMU

Metals

_ZN

_CU ×2

_MG ×2

_NA ×2

Waters ×2080

PDB id:

7coh

Name:

Oxidoreductase

Title:

Dimeric form of bovine heart cytochromE C oxidase in the fully oxidized state

Structure:

CytochromE C oxidase subunit 1. Chain: a, n. Synonym: cytochromE C oxidase polypeptide i. Engineered: yes. CytochromE C oxidase subunit 2. Chain: b, o. Synonym: cytochromE C oxidase polypeptide ii. Engineered: yes. CytochromE C oxidase subunit 3.

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Gene: mt-co1, coi, coxi, mtco1. Expressed in: bos taurus. Expression_system_taxid: 9913. Gene: mt-co3, coiii, coxiii, mtco3. Gene: cox4i1, cox4. Gene: cox5a.

Resolution:

1.30Å R-factor: 0.150 R-free: 0.170

Authors:

K.Shinzawa-Itoh,K.Muramoto

Key ref:

K.Shinzawa-Itoh et al. The 1.3-A resolution structure of bovine cytochrome c oxidase suggests a dimerization mechanism. Biochim biophys acta, .

Date:

04-Aug-20 Release date: 07-Apr-21

Protein chains

P00396  (COX1_BOVIN) -  Cytochrome c oxidase subunit 1 from Bos taurus

Seq: 514 a.a.

Struc: 513 a.a.*

Protein chains

P68530  (COX2_BOVIN) -  Cytochrome c oxidase subunit 2 from Bos taurus

Seq: 227 a.a.

Struc: 227 a.a.*

Protein chains

P00415  (COX3_BOVIN) -  Cytochrome c oxidase subunit 3 from Bos taurus

Seq: 261 a.a.

Struc: 258 a.a.

Protein chains

P00423  (COX41_BOVIN) -  Cytochrome c oxidase subunit 4 isoform 1, mitochondrial from Bos taurus

Seq: 169 a.a.

Struc: 143 a.a.

Protein chains

P00426  (COX5A_BOVIN) -  Cytochrome c oxidase subunit 5A, mitochondrial from Bos taurus

Seq: 152 a.a.

Struc: 102 a.a.

Protein chains

P00428  (COX5B_BOVIN) -  Cytochrome c oxidase subunit 5B, mitochondrial from Bos taurus

Seq: 129 a.a.

Struc: 91 a.a.

Protein chains

P07471  (CX6A2_BOVIN) -  Cytochrome c oxidase subunit 6A2, mitochondrial from Bos taurus

Seq: 97 a.a.

Struc: 72 a.a.

Protein chains

P00429  (CX6B1_BOVIN) -  Cytochrome c oxidase subunit 6B1 from Bos taurus

Seq: 86 a.a.

Struc: 75 a.a.

Protein chains

P04038  (COX6C_BOVIN) -  Cytochrome c oxidase subunit 6C from Bos taurus

Seq: 74 a.a.

Struc: 70 a.a.

Protein chains

P07470  (CX7A1_BOVIN) -  Cytochrome c oxidase subunit 7A1, mitochondrial from Bos taurus

Seq: 80 a.a.

Struc: 56 a.a.

Protein chains

P13183  (COX7B_BOVIN) -  Cytochrome c oxidase subunit 7B, mitochondrial from Bos taurus

Seq: 80 a.a.

Struc: 49 a.a.

Protein chains

P00430  (COX7C_BOVIN) -  Cytochrome c oxidase subunit 7C, mitochondrial from Bos taurus

Seq: 63 a.a.

Struc: 44 a.a.

Protein chains

P10175  (COX8B_BOVIN) -  Cytochrome c oxidase subunit 8B, mitochondrial from Bos taurus

Seq: 70 a.a.

Struc: 40 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, N, O, P: E.C.7.1.1.9 - cytochrome-c oxidase.
• Reaction: 4 Fe(II)-[cytochrome c] + O2 + 8 H⁺(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H⁺(out)

4 × Fe(II)-[cytochrome c] + O2 (Bound ligand (Het Group name = PER) corresponds exactly) + 8 × H(+)(in) = 4 × Fe(III)-[cytochrome c] + 2 × H2O + 4 × H(+)(out)

• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site