PDBsum entry 7cgd

Metal binding protein

PDB id: 7cgd

Contents

Protein chains

300 a.a.

Metals

_AG ×27

Waters ×49

PDB id:

7cgd

Name:

Metal binding protein

Title:

Silver-bound e.Coli malate dehydrogenase

Structure:

Malate dehydrogenase. Chain: a, b, c, d. Engineered: yes. Mutation: yes

Source:

Escherichia coli k-12. Organism_taxid: 83333. Strain: k-12. Gene: mdh. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.06Å R-factor: 0.215 R-free: 0.247

Authors:

H.Wang,M.Wang,H.Sun

Key ref:

H.Wang et al. Atomic differentiation of silver binding preference i protein targets: escherichia coli malate dehydrogenas paradigm. Chem sci, . PubMed id: 34123202

Date:

01-Jul-20 Release date: 23-Sep-20

Protein chains

P61889  (MDH_ECOLI) -  Malate dehydrogenase from Escherichia coli (strain K12)

Seq: 312 a.a.

Struc: 300 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.1.1.37 - malate dehydrogenase.
• Pathway: Citric acid cycle
• Reaction: (S)-malate + NAD⁺ = oxaloacetate + NADH + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site