PDBsum entry 7cf7

Transferase

PDB id: 7cf7

Contents

Protein chains

148 a.a.

Waters ×265

PDB id:

7cf7

Name:

Transferase

Title:

Crystal structure of ybea cp74 w72f

Structure:

Ribosomal RNA large subunit methyltransferase h,ribosomal RNA large subunit methyltransferase h. Chain: a, b. Synonym: 23s rrna (pseudouridine1915-n3)-methyltransferase,23s rrna m3psi1915 methyltransferase,rrna (pseudouridine-n3-)- methyltransferase rlmh. Engineered: yes. Mutation: yes

Source:

Escherichia coli k-12. Organism_taxid: 83333. Strain: k-12. Gene: rlmh, ybea, b0636, jw0631. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.62Å R-factor: 0.190 R-free: 0.218

Authors:

C.Y.Liu,C.H.Lai,S.T.D.Hsu,P.C.Lyu

Key ref:

C.Y.Liu et al. Crystal structure of ybea cp74 w72f. To be published, .

Date:

24-Jun-20 Release date: 30-Jun-21

Protein chains

P0A8I8  (RLMH_ECOLI) -  Ribosomal RNA large subunit methyltransferase H from Escherichia coli (strain K12)

Seq: 155 a.a.

Struc: 148 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.1.1.177 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase.
• Reaction: pseudouridine₁₉₁₅ in 23S rRNA + S-adenosyl-L-methionine = N₃- methylpseudouridine₁₉₁₅ in 23S rRNA + S-adenosyl-L-homocysteine + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site