PDBsum entry 7apl

Transferase

PDB id: 7apl

Contents

Protein chain

374 a.a.

Ligands

GOL ×2

Metals

_ZN

Waters ×173

PDB id:

7apl

Name:

Transferase

Title:

tRNA-guanine transglycosylase g87c mutant spin-labeled with mtsl

Structure:

Queuine tRNA-ribosyltransferase. Chain: a. Synonym: guanine insertion enzyme,tRNA-guanine transglycosylase. Engineered: yes. Mutation: yes

Source:

Zymomonas mobilis subsp. Mobilis (strain atcc 31821 / zm4 / cp4). Organism_taxid: 264203. Strain: atcc 31821 / zm4 / cp4. Gene: tgt, zmo0363. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.99Å R-factor: 0.174 R-free: 0.209

Authors:

D.Nguyen,A.Heine,G.Klebe

Key ref:

D.Abdullin et al. Unraveling a ligand-Induced twist of a homodimeric en pulsed electron-Electron double resonance. To be published, . PubMed id: 34387025

Date:

18-Oct-20 Release date: 28-Oct-20

Protein chain

P28720  (TGT_ZYMMO) -  Queuine tRNA-ribosyltransferase from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

Seq: 386 a.a.

Struc: 374 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.4.2.29 - tRNA-guanosine(34) preQ1 transglycosylase.
• Reaction: 7-aminomethyl-7-carbaguanine + guanosine₃₄ in tRNA = 7-aminomethyl-7- carbaguanosine₃₄ in tRNA + guanine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site