PDBsum entry 7ad0

Ligase

PDB id: 7ad0

Contents

Protein chains

(+ 0 more) 90 a.a.

(+ 0 more) 14 a.a.

Waters ×165

PDB id:

7ad0

Name:

Ligase

Title:

X-ray structure of mdm2 with modified p53 peptide

Structure:

E3 ubiquitin-protein ligase mdm2. Chain: a, b, c, d, e, f. Synonym: double minute 2 protein,hdm2,oncoprotein mdm2,ring-type e3 ubiquitin transferase mdm2,p53-binding protein mdm2. Engineered: yes. Modified p53 peptide. Chain: h, i, j, k, l, m. Engineered: yes. Other_details: this short chain is a modified p53 peptide. 475 is a

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mdm2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.07Å R-factor: 0.190 R-free: 0.239

Authors:

A.Twarda-Clapa,P.Fortuna,P.Grudnik,G.Dubin,L.Berlicki,T.A.Holak

Key ref:

P.Fortuna et al. (2020). Systematic "foldamerization" of peptide inhibiting p53-Mdm2/X interactions by the incorporation of trans cis-2-Aminocyclopentanecarboxylic acid residues. European journal of, 208, 12814.

Date:

13-Sep-20 Release date: 28-Oct-20

Protein chains

Q00987  (MDM2_HUMAN) -  E3 ubiquitin-protein ligase Mdm2 from Homo sapiens

Seq: 491 a.a.

Struc: 90 a.a.

Protein chains

No UniProt id for this chain

Struc: 14 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine