PDBsum entry 7ab6

Fluorescent protein

PDB id: 7ab6

Contents

Protein chains

106 a.a.

Ligands

FMN ×2

GOL

SO4

Waters ×189

PDB id:

7ab6

Name:

Fluorescent protein

Title:

Structure of chloroflexus aggregans flavin based fluorescent protein (cagfbfp) i52t variant

Structure:

Multi-sensor hybrid histidine kinase. Chain: a, b. Engineered: yes

Source:

Chloroflexus aggregans (strain md-66 / dsm 9485). Organism_taxid: 326427. Strain: md-66 / dsm 9485. Gene: cagg_3753. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.90Å R-factor: 0.181 R-free: 0.213

Authors:

A.Remeeva,V.Nazarenko,K.Kovalev,I.Gushchin

Key ref:

k.rollen et al. (2021). The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins. J.Biol.Chem., 0, 100662-100662. PubMed id: 33862085

Date:

06-Sep-20 Release date: 21-Apr-21

Protein chains

B8GAY9  (B8GAY9_CHLAD) -  histidine kinase from Chloroflexus aggregans (strain MD-66 / DSM 9485)

Seq: 913 a.a.

Struc: 106 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.13.3 - histidine kinase.
• Reaction: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

ATP + protein L-histidine = ADP (Bound ligand (Het Group name = FMN) matches with 61.11% similarity) + protein N-phospho-L-histidine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J.Biol.Chem. 0:100662-100662 (2021)

PubMed id: 33862085

The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.

k.rollen, j.granzin, a.remeeva, m.d.davari, t.gensch, v.v.nazarenko, k.kovalev, a.bogorodskiy, v.borshchevskiys.hemmer, u.schwaneberg, v.gordeliy, k.e.jaeger, r.batra-safferling, i.gushchin, u.krauss.

ABSTRACT

No abstract given.