PDBsum entry 7a1c

Ligase

PDB id: 7a1c

Contents

Protein chains

256 a.a.

Ligands

DMS ×3

Waters ×641

PDB id:

7a1c

Name:

Ligase

Title:

Ldtmt2 with covalent adduct derived from n-thio-beta-lactam 1a

Structure:

L,d-transpeptidase 2. Chain: a, b. Synonym: ldt 2,ldt(mt2). Engineered: yes. Other_details: the construct used here contains two domains: domain-b (ig-like) and domain-c (catalytic transpeptidase domain), residue range 149-408 of the full length protein.

Source:

Mycobacterium tuberculosis (strain atcc 25618 / h37rv). Organism_taxid: 83332. Strain: atcc 25618 / h37rv. Gene: ldtb, lpps, rv2518c, rvbd_2518c, p425_02624. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.77Å R-factor: 0.180 R-free: 0.208

Authors:

R.Schnell,E.M.Steiner

Key ref:

G.Martelli et al. N-Thio-Beta-Lactams targeting l,D-Transpeptidase-2, W activity against drug-Resistant strains of mycobacter tuberculosis.. Cell chem biol, . PubMed id: 33826941

Date:

12-Aug-20 Release date: 21-Apr-21

Protein chains

I6Y9J2  (LDT2_MYCTU) -  L,D-transpeptidase 2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 408 a.a.

Struc: 256 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.- - ?????