PDBsum entry 7a0c

Metal binding protein

PDB id: 7a0c

Contents

Protein chains

498 a.a.

Ligands

QTT ×2

ACT ×9

GOL ×9

Metals

_MG ×5

_CL

_FE ×2

Waters ×795

PDB id:

7a0c

Name:

Metal binding protein

Title:

X-ray structure of nika from escherichia coli in complex with fe-6- me2-bpmcn

Structure:

Nickel-binding periplasmic protein. Chain: a, b. Engineered: yes

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Strain: k12. Gene: nika, b3476, jw3441. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.90Å R-factor: 0.184 R-free: 0.222

Authors:

C.Cavazza,S.Menage

Key ref:

S.Lopez et al. (2020). A Selective Sulfide Oxidation Catalyzed by Heterogeneous Artificial Metalloenzymes Iron@NikA. Chemistry, 26, 16633-16638. PubMed id: 33079395 DOI: 10.1002/chem.202003746

Date:

07-Aug-20 Release date: 04-Nov-20

Protein chains

P33590  (NIKA_ECOLI) -  Nickel-binding periplasmic protein from Escherichia coli (strain K12)

Seq: 524 a.a.

Struc: 498 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1002/chem.202003746

Chemistry 26:16633-16638 (2020)

PubMed id: 33079395

A Selective Sulfide Oxidation Catalyzed by Heterogeneous Artificial Metalloenzymes Iron@NikA.

S.Lopez, C.Marchi-Delapierre, C.Cavazza, S.Ménage.

ABSTRACT

Performing a heterogeneous catalysis with proteins is still a challenge. Herein, we demonstrate the importance of cross-linked crystals for sulfoxide oxidation by an artificial enzyme. The biohybrid consists of the insertion of an iron complex into a NikA protein crystal. The heterogeneous catalysts displays a better efficiency-with higher reaction kinetics, a better stability and expand the substrate scope compared to its solution counterpart. Designing crystalline artificial enzymes represents a good alternative to soluble or supported enzymes for the future of synthetic biology.