PDBsum entry 6zij

Oxidoreductase

PDB id: 6zij

Contents

Protein chains

(+ 0 more) 277 a.a.

Ligands

GOL ×5

EDO ×2

OXY ×6

Metals

_MG ×3

_CU ×24

Waters ×964

PDB id:

6zij

Name:

Oxidoreductase

Title:

Crystal structure of two-domain laccase mutant r240h from streptomyces griseoflavus

Structure:

Two-domain laccase. Chain: a, b, c, d, e, f. Engineered: yes. Mutation: yes

Source:

Streptomyces griseoflavus. Organism_taxid: 35619. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.60Å R-factor: 0.147 R-free: 0.181

Authors:

A.G.Gabdulkhakov,T.V.Tishchenko,I.A.Kolyadenko

Key ref:

A.Gabdulkhakov et al. The role of positive charged residue in the proton-Tr mechanism of two-Domain laccase from streptomyces griseoflavus ac-993.. J biomol struct dyn, . PubMed id: 33870857

Date:

26-Jun-20 Release date: 05-May-21

Protein chains

A0A0M4FJ81  (A0A0M4FJ81_9ACTN) -  Two-domain laccase from Streptomyces griseoflavus

Seq: 322 a.a.

Struc: 277 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.10.3.2 - laccase.
• Reaction: 4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O

4 × hydroquinone + O2 (Bound ligand (Het Group name = OXY) corresponds exactly) = 4 × benzosemiquinone + 2 × H2O

• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site