PDBsum entry 6zcc

Hydrolase

PDB id: 6zcc

Contents

Protein chain

291 a.a.

Ligands

OEH

ACT

Metals

_CA ×4

Waters ×312

PDB id:

6zcc

Name:

Hydrolase

Title:

X-ray structure of the haloalkane dehalogenase hob (halotag7-based oligonucleotide binder) labeled with a chloroalkane- tetramethylrhodamine fluorophore substrate

Structure:

Haloalkane dehalogenase. Chain: a. Engineered: yes

Source:

Rhodococcus sp.. Organism_taxid: 1831. Gene: dhaa. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.52Å R-factor: 0.191 R-free: 0.224

Authors:

M.Tarnawski,K.Johnsson,J.Hiblot

Key ref:

M.Tarnawski et al. X-Ray structure of the haloalkane dehalogenase hob (halotag7-Based oligonucleotide binder) labeled with chloroalkane-Tetramethylrhodamine fluorophore substra. To be published, . PubMed id: 34339177

Date:

10-Jun-20 Release date: 21-Apr-21

Protein chain

P0A3G3  (DHAA_RHOSO) -  Haloalkane dehalogenase from Rhodococcus sp

Seq: 293 a.a.

Struc: 291 a.a.*

* PDB and UniProt seqs differ at 25 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.8.1.5 - haloalkane dehalogenase.
• Reaction: 1-haloalkane + H2O = a halide anion + a primary alcohol + H⁺

1-haloalkane + H2O = halide anion + primary alcohol (Bound ligand (Het Group name = ACT) matches with 40.00% similarity) + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site