PDBsum entry 6z2f

Biosynthetic protein

PDB id

6z2f

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Contents

			Protein chains

					469 a.a.


					475 a.a.

			Ligands

					UD1 ×2

			Waters ×701

PDB id:

6z2f

Links

Name:

Biosynthetic protein

Title:

Crystal structure of human agx1 mutant complexed with udpglcnac

Structure:

Udp-n-acetylhexosamine pyrophosphorylase. Chain: a. Synonym: antigen x,agx,sperm-associated antigen 2. Engineered: yes. Udp-n-acetylhexosamine pyrophosphorylase. Chain: b. Synonym: antigen x,agx,sperm-associated antigen 2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: uap1, spag2. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

1.70Å

R-factor:

0.189

R-free:

0.226

Authors:

X.Chen,D.M.F.Van Aalten

Key ref:

X.Chen and d.m.f.van aalten Crystal structure of human agx1 mutant complexed with udpglcnac. To be published, .

Date:

15-May-20

Release date:

26-May-21

PROCHECK

	Headers

	References

Protein chain

Q16222 (UAP1_HUMAN) - UDP-N-acetylhexosamine pyrophosphorylase from Homo sapiens

Seq: Struc:

Seq: Struc:					522 a.a. 469 a.a.*

Protein chain

Q16222 (UAP1_HUMAN) - UDP-N-acetylhexosamine pyrophosphorylase from Homo sapiens

Seq: Struc:

Seq: Struc:					522 a.a. 475 a.a.*

Key:

PfamA domain

Secondary structure

* PDB and UniProt seqs differ at 3 residue positions (black crosses)



		Enzyme reactions

Enzyme class 2:

Chains A, B: E.C.2.7.7.23 - UDP-N-acetylglucosamine diphosphorylase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

UDP-N-acetylglucosamine Biosynthesis

Reaction:

N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H⁺ = UDP-N-acetyl- alpha-D-glucosamine + diphosphate

N-acetyl-alpha-D-glucosamine 1-phosphate	+	UTP	+	H(+) Bound ligand (Het Group name = UD1) matches with 58.14% similarity	=	UDP-N-acetyl- alpha-D-glucosamine	+	diphosphate

Enzyme class 3:

Chains A, B: E.C.2.7.7.83 - UDP-N-acetylgalactosamine diphosphorylase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

N-acetyl-alpha-D-galactosamine 1-phosphate + UTP + H⁺ = UDP-N-acetyl- alpha-D-galactosamine + diphosphate

N-acetyl-alpha-D-galactosamine 1-phosphate	+	UTP	+	H(+) Bound ligand (Het Group name = UD1) matches with 58.14% similarity	=	UDP-N-acetyl- alpha-D-galactosamine	+	diphosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site