PDBsum entry 6yvq

Ligase

PDB id: 6yvq

Contents

Protein chain

295 a.a.

Ligands

SO4 ×2

EDO ×3

Waters ×280

PDB id:

6yvq

Name:

Ligase

Title:

Crystal structure of saicar synthetase (purc) from mycobacterium abscessus in apo form

Structure:

Phosphoribosylaminoimidazole-succinocarboxamide synthase. Chain: a. Synonym: saicar synthetase. Engineered: yes

Source:

Mycobacteroides abscessus (strain atcc 19977 / dsm 44196 / cip 104536 / jcm 13569 / nctc 13031 / tmc 1543). Organism_taxid: 561007. Strain: atcc 19977 / dsm 44196 / cip 104536 / jcm 13569 / nctc 13031 / tmc 1543. Gene: purc, mab_0689. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.48Å R-factor: 0.164 R-free: 0.197

Authors:

S.E.Thomas,S.Charoensutthivarakul,A.G.Coyne,C.Abell,T.L.Blundell

Key ref:

S.E.Thomas et al. Fragment-Based design and discovery of novel inhibito targeting mycobacterium abscessus saicar synthetase (. To be published, . PubMed id: 35037462

Date:

28-Apr-20 Release date: 12-May-21

Protein chain

B1MHW4  (PUR7_MYCA9) -  Phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CCUG 20993 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543 / L948)

Seq: 297 a.a.

Struc: 295 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.2.6 - phosphoribosylaminoimidazolesuccinocarboxamide synthase.
• Pathway: Purine Biosynthesis (late stages)
• Reaction: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4- carboxamido]succinate + ADP + phosphate + 2 H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site