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PDBsum entry 6yvh
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RNA binding protein
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PDB id
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6yvh
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Contents |
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274 a.a.
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49 a.a.
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162 a.a.
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44 a.a.
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PDB id:
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| Name: |
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RNA binding protein
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Title:
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Cwc22-cwc27-eif4a3 complex
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Structure:
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Pre-mRNA-splicing factor cwc22 homolog. Chain: a, b, d, f. Synonym: nucampholin homolog,fsapb. Engineered: yes. Spliceosome-associated protein cwc27 homolog. Chain: c, i, e, g. Synonym: antigen ny-co-10,probable inactive peptidyl-prolyl cis-trans isomerase cwc27 homolog,ppiase cwc27,serologically defined colon cancer antigen 10.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: cwc22, kiaa1604, ncm. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: cwc27, sdccag10, unq438/pro871. Gene: eif4a3, ddx48, kiaa0111.
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Resolution:
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3.19Å
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R-factor:
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0.233
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R-free:
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0.263
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Authors:
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J.Basquin,V.Busetto,H.Lehir,E.Conti
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Key ref:
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V.Busetto
et al.
(2020).
Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes.
Nucleic Acids Res,
48,
5670-5683.
PubMed id:
DOI:
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Date:
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28-Apr-20
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Release date:
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13-May-20
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PROCHECK
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Headers
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References
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Q9HCG8
(CWC22_HUMAN) -
Pre-mRNA-splicing factor CWC22 homolog from Homo sapiens
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Seq:
Struc:
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908 a.a.
274 a.a.
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Q6UX04
(CWC27_HUMAN) -
Spliceosome-associated protein CWC27 homolog from Homo sapiens
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Seq:
Struc:
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472 a.a.
49 a.a.
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Enzyme class:
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Chains H, J, K, L:
E.C.3.6.4.13
- Rna helicase.
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Reaction:
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ATP + H2O = ADP + phosphate + H+
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ATP
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+
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H2O
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=
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ADP
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+
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phosphate
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+
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H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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Nucleic Acids Res
48:5670-5683
(2020)
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PubMed id:
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Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes.
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V.Busetto,
I.Barbosa,
J.Basquin,
Ã.‰.Marquenet,
R.Hocq,
M.Hennion,
J.A.Paternina,
A.Namane,
E.Conti,
O.Bensaude,
H.Le Hir.
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ABSTRACT
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Human CWC27 is an uncharacterized splicing factor and mutations in its gene are
linked to retinal degeneration and other developmental defects. We identify the
splicing factor CWC22 as the major CWC27 partner. Both CWC27 and CWC22 are
present in published Bact spliceosome structures, but no interacting domains are
visible. Here, the structure of a CWC27/CWC22 heterodimer bound to the exon
junction complex (EJC) core component eIF4A3 is solved at 3Ã…-resolution.
According to spliceosomal structures, the EJC is recruited in the C complex,
once CWC27 has left. Our 3D structure of the eIF4A3/CWC22/CWC27 complex is
compatible with the Bact spliceosome structure but not with that of the C
complex, where a CWC27 loop would clash with the EJC core subunit Y14. A
CWC27/CWC22 building block might thus form an intermediate landing platform for
eIF4A3 onto the Bact complex prior to its conversion into C complex. Knock-down
of either CWC27 or CWC22 in immortalized retinal pigment epithelial cells
affects numerous common genes, indicating that these proteins cooperate,
targeting the same pathways. As the most up-regulated genes encode factors
involved in inflammation, our findings suggest a possible link to the retinal
degeneration associated with CWC27 deficiencies.
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