PDBsum entry 6ygo

Transferase

PDB id: 6ygo

Contents

Protein chain

373 a.a.

Ligands

GOL ×4

Metals

_ZN

Waters ×362

PDB id:

6ygo

Name:

Transferase

Title:

Tgt w95f mutant labelled mit 5f-trp crystallised at ph 8.5

Structure:

Queuine tRNA-ribosyltransferase. Chain: a. Synonym: guanine insertion enzyme,tRNA-guanine transglycosylase. Engineered: yes. Mutation: yes

Source:

Zymomonas mobilis subsp. Mobilis (strain atcc 31821 / zm4 / cp4). Organism_taxid: 264203. Strain: atcc 31821 / zm4 / cp4. Gene: tgt, zmo0363. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.26Å R-factor: 0.124 R-free: 0.145

Authors:

A.Nguyen,A.Heine,G.Klebe

Date:

27-Mar-20 Release date: 08-Apr-20

Protein chain

P28720  (TGT_ZYMMO) -  Queuine tRNA-ribosyltransferase from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

Seq: 386 a.a.

Struc: 373 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.4.2.29 - tRNA-guanosine(34) preQ1 transglycosylase.
• Reaction: 7-aminomethyl-7-carbaguanine + guanosine₃₄ in tRNA = 7-aminomethyl-7- carbaguanosine₃₄ in tRNA + guanine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site