PDBsum entry 6y9m

Viral protein

PDB id: 6y9m

Contents

Protein chains

195 a.a.

Ligands

NAG-NAG ×6

NAG-NAG-BMA-FUC

NAG-NAG-BMA

PO4 ×5

SO4

Waters ×7

PDB id:

6y9m

Name:

Viral protein

Title:

Crystal structure of tswv glycoprotein n ectodomain (sgn)

Structure:

Glycoprotein. Chain: a, b, c, d. Engineered: yes

Source:

Tomato spotted wilt virus (strain brazilian br- 01). Organism_taxid: 36413. Gene: gn, gc. Expressed in: trichoplusia ni. Expression_system_taxid: 7111

Resolution:

3.40Å R-factor: 0.261 R-free: 0.283

Authors:

M.Dessau,Y.Bahat

Key ref:

Y.Bahat et al. (2020). Crystal structure of tomato spotted wilt virus G_N reveals a dimer complex formation and evolutionary link to animal-infecting viruses. Proc Natl Acad Sci U S A, 117, 26237-26244. PubMed id: 33020295 DOI: 10.1073/pnas.2004657117

Date:

10-Mar-20 Release date: 30-Sep-20

Protein chains

P36291  (GP_TSWV1) -  Envelopment polyprotein from Tomato spotted wilt virus (strain Brazilian Br-01)

Seq: 1135 a.a.

Struc: 195 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1073/pnas.2004657117

Proc Natl Acad Sci U S A 117:26237-26244 (2020)

PubMed id: 33020295

Crystal structure of tomato spotted wilt virus G_N reveals a dimer complex formation and evolutionary link to animal-infecting viruses.

Y.Bahat, J.Alter, M.Dessau.

ABSTRACT

Tospoviridae is a family of enveloped RNA plant viruses that infect many field crops, inflicting a heavy global economic burden. These tripartite, single-stranded, negative-sense RNA viruses are transmitted from plant to plant by thrips as the insect vector. The medium (M) segment of the viral genome encodes two envelope glycoproteins, G_N and G_C, which together form the envelope spikes. G_C is considered the virus fusogen, while the accompanying G_N protein serves as an attachment protein that binds to a yet unknown receptor, mediating the virus acquisition by the thrips carrier. Here we present the crystal structure of glycoprotein N (G_N) from the tomato spotted wilt virus (TSWV), a representative member of the Tospoviridae family. The structure suggests that G_N is organized as dimers on TSWV's outer shell. Our structural data also suggest that this dimerization is required for maintaining G_N structural integrity. Although the structure of the TSWV G_N is different from other bunyavirus G_N proteins, they all share similar domain connectivity that resembles glycoproteins from unrelated animal-infecting viruses, suggesting a common ancestor for these accompanying proteins.