PDBsum entry 6x9f

Ligase

PDB id: 6x9f

Contents

Protein chains

(+ 2 more) 304 a.a.

Ligands

UXP ×8

EDO ×32

DMS ×13

Metals

_CL ×5

Waters ×586

PDB id:

6x9f

Name:

Ligase

Title:

Pseudomonas aeruginosa murc with az8074

Structure:

Udp-n-acetylmuramate--l-alanine ligase. Chain: a, b, c, d, e, f, g, h. Synonym: udp-n-acetylmuramoyl-l-alanine synthetase. Engineered: yes

Source:

Pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1). Organism_taxid: 208964. Strain: atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1. Gene: murc, pa4411. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

2.35Å R-factor: 0.184 R-free: 0.219

Authors:

P.S.Horanyi,S.J.Mayclin,T.F.Durand-Reville,D.D.Lorimer,T.E.Edwards, Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

P.S.Horanyi et al. Pseudomonas aeruginosa murc with az8074. To be published, .

Date:

02-Jun-20 Release date: 02-Sep-20

Protein chains

Q9HW02  (MURC_PSEAE) -  UDP-N-acetylmuramate--L-alanine ligase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 480 a.a.

Struc: 304 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.3.2.8 - UDP-N-acetylmuramate--L-alanine ligase.
• Pathway: Peptidoglycan Biosynthesis (Part 1)
• Reaction: UDP-N-acetyl-alpha-D-muramate + L-alanine + ATP = UDP-N-acetyl-alpha-D- muramoyl-L-alanine + ADP + phosphate + H⁺

UDP-N-acetyl-alpha-D-muramate + L-alanine (Bound ligand (Het Group name = EDO) matches with 42.86% similarity) + ATP = UDP-N-acetyl-alpha-D- muramoyl-L-alanine + ADP + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site